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用于区分两种营养变异链球菌(缺陷链球菌和毗邻链球菌)的酶活性分析。

Analysis of enzymatic activities for differentiation of two species of nutritionally variant streptococci, Streptococcus defectivus and Streptococcus adjacens.

作者信息

Beighton D, Homer K A, Bouvet A, Storey A R

机构信息

RCS Department of Dental Sciences, Faculty of Clinical Dentistry, King's College School of Medicine and Dentistry, London, United Kingdom.

出版信息

J Clin Microbiol. 1995 Jun;33(6):1584-7. doi: 10.1128/jcm.33.6.1584-1587.1995.

Abstract

Strains of nutritionally variant streptococci, Streptococcus defectivus (n = 10) and Streptococcus adjacens (n = 20), were studied for the production of glycosidic and proteolytic enzyme activities. S. defectivus strains produced neuraminidase and alpha-fucosidase, while S. adjacens strains produced only neuraminidase. The S. adjacens strains produced a very wide range of proteolytic activities with the ability to hydrolyze the majority of aminopeptidase substrates tested, while S. defectivus strains hydrolyzed only a minority of aminopeptidase substrates. These data provide additional phenotypic characteristics which may be useful in distinguishing between these two species and suggest that they may have different nutritional requirements.

摘要

对营养变异链球菌菌株,即缺陷链球菌(n = 10)和毗邻链球菌(n = 20)进行了糖苷酶和蛋白水解酶活性产生情况的研究。缺陷链球菌菌株产生神经氨酸酶和α-岩藻糖苷酶,而毗邻链球菌菌株仅产生神经氨酸酶。毗邻链球菌菌株产生非常广泛的蛋白水解活性,能够水解所测试的大多数氨肽酶底物,而缺陷链球菌菌株仅水解少数氨肽酶底物。这些数据提供了额外的表型特征,可能有助于区分这两个菌种,并表明它们可能有不同的营养需求。

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