van Soolingen D, Bauer J, Ritacco V, Leão S C, Pavlik I, Vincent V, Rastogi N, Gori A, Bodmer T, Garzelli C, Garcia M J
Diagnostic Laboratory of Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands.
J Clin Microbiol. 1998 Oct;36(10):3051-4. doi: 10.1128/JCM.36.10.3051-3054.1998.
Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.
鸟分枝杆菌已成为一种主要的人类病原体，这主要归因于艾滋病疫情的出现。使用插入序列IS1245作为探针的限制性片段长度多态性（RFLP）分型，为鸟分枝杆菌相关感染的分子流行病学研究提供了一个强大的工具，并将有助于对动物和环境宿主中鸟分枝杆菌感染源进行有充分依据的研究。该技术的标准化使得IS1245 RFLP模式能够实现计算机化，以便在本地层面进行比较，并建立鸟分枝杆菌DNA指纹数据库用于实验室间比较。此外，通过整合来自广泛来源的鸟分枝杆菌复合群分离株的国际DNA分型结果，关于这种主要分枝杆菌感染病原体流行病学的长期问题将得到解答。
