Molenaar M, Roose J, Peterson J, Venanzi S, Clevers H, Destrée O
NIOB/Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Mech Dev. 1998 Jul;75(1-2):151-4. doi: 10.1016/s0925-4773(98)00085-9.
The recent discovery that the HMG box transcription factor XTCF-3 is involved in early axis specification in Xenopus laevis (Molenaar, M., van de Wetering, M., Oosterwegel, M., Peterson-Maduro, J. Godsave, S., Korinek, V., Roose, J., Destree, O., Clevers, H., 1996. XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos. Cell 86, 391-399) led us to search for other members of the TCF/LEF family in this species. A newly identified HMG box factor was cloned with highest homology to human LEF-1, called XLEF-1. Unlike XTcf-3, XLef-1 is not expressed maternally, but its transcripts become detectable directly after the mid blastula transition (MBT). At later stages, both genes are expressed in the central nervous system (CNS), eyes, otic vesicles, head mesenchyme, neural crest and derivatives, branchial arches, developing heart, tailbud and limb buds. The expression pattern of Lef-1 during later stages of development is evolutionarily conserved.
最近发现HMG盒转录因子XTCF-3参与非洲爪蟾早期轴的特化过程（莫伦纳尔，M.，范德韦特林，M.，奥斯特韦格尔，M.，彼得森 - 马杜罗，J.，戈德萨夫，S.，科里内克，V.，罗斯，J.，德斯特雷，O.，克勒弗斯，H.，1996年。XTcf-3转录因子介导非洲爪蟾胚胎中β-连环蛋白诱导的轴形成。《细胞》86卷，391 - 399页），这促使我们在该物种中寻找TCF/LEF家族的其他成员。一个新鉴定的与人类LEF-1具有最高同源性的HMG盒因子被克隆出来，称为XLEF-1。与XTcf-3不同，XLef-1不是母源表达，但其转录本在囊胚中期转换（MBT）后可直接检测到。在发育后期，这两个基因都在中枢神经系统（CNS）、眼睛、耳泡、头部间充质、神经嵴及其衍生物、鳃弓、发育中的心脏、尾芽和肢芽中表达。Lef-1在发育后期的表达模式在进化上是保守的。
