Department of Oncology, The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, Chigang, Guangzhou 510305, China.
Mol Biotechnol. 2012 Sep;52(1):59-67. doi: 10.1007/s12033-011-9474-7.
Increased expression of cell adhesion molecule CD44v6, integrin-β1, carbohydrate antigen 199 (CA199), and carcinoembryonic antigen (CEA) are closely associated with the progression and metastasis of numerous cancers. In this study, peripheral blood mononuclear cell (PBMC) and serum samples were collected from 37 pancreatic cancer patients and 12 healthy people. A novel triplex TaqMan real-time reverse transcription polymerase chain reaction assay was used to measure the expression levels of CD44v6 and integrin-β1 gene in PBMCs, while chemiluminescence and enzyme-linked immunosorbent assay were used to measure the levels of CA199 and CEA expression in serum. The results showed that both the levels of CD44v6 and integrin-β1 expression had significant correlation with clinical stage, lymph node, and liver metastasis of pancreatic cancer (P < 0.05). Age, tumor size, tumor differentiation, clinical stage, lymph nodes, and liver metastasis were significantly associated with the levels of CA199 and CEA expression (P < 0.05). The levels of CD44v6, integrin-β1, CA199, and CEA expression in the patients prior cryosurgery and chemotherapy were significantly higher than those in the control group (P < 0.05), whereas no significant difference was found between the patients 1 month post cryosurgery and control group (P > 0.05). The expression levels of CD44v6, integrin-β1, CA199, and CEA in the patients 1 month post cryosurgery were significantly lower than those in the patients prior cryosurgery (P < 0.05). Interestingly, no significant difference was found for the CD44v6, integrin-β1, CA199, and CEA levels between the patients prior and post-chemotherapy (P > 0.05). The higher expression of CD44v6, integrin-β1, CA199, and CEA are closely related to the progression and metastasis of pancreatic cancer and may play a important role in the curative evaluation of cryosurgery of pancreatic cancer.
细胞黏附分子 CD44v6、整合素-β1、碳水化合物抗原 199（CA199）和癌胚抗原（CEA）的表达增加与许多癌症的进展和转移密切相关。在这项研究中，收集了 37 名胰腺癌患者和 12 名健康人的外周血单核细胞（PBMC）和血清样本。使用新型三重 TaqMan 实时逆转录聚合酶链反应测定法测量 PBMC 中 CD44v6 和整合素-β1 基因的表达水平，同时使用化学发光和酶联免疫吸附试验测量血清中 CA199 和 CEA 的表达水平。结果表明，CD44v6 和整合素-β1 的表达水平与胰腺癌的临床分期、淋巴结和肝转移均有显著相关性（P<0.05）。年龄、肿瘤大小、肿瘤分化、临床分期、淋巴结和肝转移与 CA199 和 CEA 的表达水平显著相关（P<0.05）。冷冻治疗前和化疗前患者的 CD44v6、整合素-β1、CA199 和 CEA 表达水平明显高于对照组（P<0.05），而冷冻治疗后 1 个月患者与对照组之间无显著差异（P>0.05）。冷冻治疗后 1 个月患者的 CD44v6、整合素-β1、CA199 和 CEA 表达水平明显低于冷冻治疗前（P<0.05）。有趣的是，化疗前后患者的 CD44v6、整合素-β1、CA199 和 CEA 水平无显著差异（P>0.05）。CD44v6、整合素-β1、CA199 和 CEA 的高表达与胰腺癌的进展和转移密切相关，可能在胰腺癌冷冻治疗的疗效评估中发挥重要作用。
