Nagaprasad V, Singh M
Biomedical Engineering Division, Indian Institute of Technology, Madras.
Clin Hemorheol Microcirc. 1998 Jul;18(4):273-84.
The hemorheological and morphological changes in blood during storage under standardised conditions for 35 days were analysed by sequential determination of the variability of aggregation, deformability and shape of erythrocytes. The shape analysis was carried out by shape descriptors based on the projected area and perimeter, as measured by processing of erythrocyte images obtained from blood smears. The aggregation of erythrocytes was analysed from the data on the sequential variation of transmitted laser intensity after passing through the erythrocyte suspension. Similarly, the deformability of erythrocytes was determined in terms of passage time as obtained by analysis of flow of erythrocyte suspension through the cellulose membrane. The results show that the erythrocyte aggregation parameters and shape descriptors show significant variation, whereas the deformability is reduced (up to 5%) compared with that of the fresh samples. These variations may explain the observed changes in blood viscosity and viability of erythrocytes after their infusion in to the cardiovascular system.
通过对红细胞聚集性、变形性和形状变异性的连续测定，分析了在标准化条件下储存35天期间血液的血液流变学和形态学变化。形状分析是通过基于投影面积和周长的形状描述符进行的，这些数据是通过处理从血涂片获得的红细胞图像测量得到的。根据激光透过红细胞悬液后透射激光强度的连续变化数据，分析红细胞的聚集性。同样，通过分析红细胞悬液流经纤维素膜的情况所获得的通过时间来确定红细胞的变形性。结果表明，红细胞聚集参数和形状描述符有显著变化，而与新鲜样本相比，变形性降低（高达5%）。这些变化可能解释了红细胞输注到心血管系统后观察到的血液粘度和红细胞活力的变化。
