Babu N, Singh M
Biomedical Engineering Division, Indian Institute of Technology, Madras-600 036, India.
Clin Hemorheol Microcirc. 2004;31(4):273-80.
The hemorheological and morphological changes in blood samples of diabetic patients, with varying levels of hyperglycemia but normal cholesterol concentration, are determined and are compared with healthy subjects. The shape analysis is carried out by shape descriptors based on projected area, perimeter and form factor, as measured by processing of images of erythrocytes. The aggregation and deformability of erythrocytes are measured by sequential variation of the transmitted laser light after passing through the erythrocytes suspension in plasma and passage of erythrocytes suspension in physiological saline through cellulose membrane, respectively. The results show that with the increase of glucose concentration the erythrocytes aggregation is enhanced and deformability is reduced in diabetic patients compared to that of healthy subjects. The shape parameters, which quantify the changes in erythrocytes in diabetes, show significant deviation from the shape of normal cells. A significant increase in the perimeter to area ratio and form factor in hyperglycemia also corresponds to highly significant increase in filtration time (decrease in deformability).
对血糖水平各异但胆固醇浓度正常的糖尿病患者血液样本的血液流变学和形态学变化进行了测定，并与健康受试者进行了比较。形状分析是通过基于投影面积、周长和形状因子的形状描述符来进行的，这些参数是通过处理红细胞图像测量得到的。红细胞的聚集性和可变形性分别通过激光透过血浆中红细胞悬液后的顺序变化以及生理盐水红细胞悬液透过纤维素膜后的变化来测量。结果表明，与健康受试者相比，糖尿病患者随着葡萄糖浓度的升高，红细胞聚集增强且可变形性降低。量化糖尿病患者红细胞变化的形状参数显示出与正常细胞形状的显著偏差。高血糖状态下周长与面积比和形状因子的显著增加也对应着过滤时间的高度显著增加（可变形性降低）。
