Mise H, Sagawa N, Matsumoto T, Yura S, Nanno H, Itoh H, Mori T, Masuzaki H, Hosoda K, Ogawa Y, Nakao K
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Japan.
J Clin Endocrinol Metab. 1998 Sep;83(9):3225-9. doi: 10.1210/jcem.83.9.5117.
Preeclampsia (PE) is a hypertensive disorder, which develops in late pregnancy and is usually associated with placental hypoxia and dysfunction. We have recently demonstrated that leptin is a novel placenta-derived hormone in humans and suggested its significance in human pregnancy (see Ref. 19). To explore the changes in the leptin production in placenta in PE, we measured the plasma leptin level and placental leptin messenger RNA expression in pregnant women with PE. Plasma leptin levels in preeclamptic women were elevated significantly, compared with gestational age- and body mass index-matched normal pregnant women (P < 0.0001). Plasma leptin levels in the severe PE group were significantly higher than those in the mild PE group (P < 0.0001). Plasma leptin levels in preeclamptic women were reduced, soon after the placental delivery, to those expected for their body mass indices. Northern blot analysis revealed that leptin messenger RNA levels are increased in the placentas from preeclamptic women, compared with normal pregnant women. Leptin secretion was increased significantly in a human trophoblastic cell line (BeWo cells) cultured under hypoxic conditions (5% O2), compared with those cultured under standard conditions (20% O2; P < 0.01). The present study demonstrated that placental production of leptin is augmented in severe PE, probably because of placental hypoxia, thereby suggesting the possible significance of leptin as a marker of placental hypoxia in severe PE.
子痫前期（PE）是一种高血压疾病，在妊娠晚期发病，通常与胎盘缺氧和功能障碍有关。我们最近证明，瘦素是一种新的人类胎盘衍生激素，并指出了其在人类妊娠中的重要性（见参考文献19）。为了探究子痫前期患者胎盘中瘦素产生的变化，我们测量了子痫前期孕妇的血浆瘦素水平和胎盘瘦素信使核糖核酸表达。与孕周和体重指数匹配的正常孕妇相比，子痫前期妇女的血浆瘦素水平显著升高（P<0.0001）。重度子痫前期组的血浆瘦素水平显著高于轻度子痫前期组（P<0.0001）。胎盘娩出后不久，子痫前期妇女的血浆瘦素水平降至与其体重指数预期相符的水平。Northern印迹分析显示，与正常孕妇相比，子痫前期妇女胎盘的瘦素信使核糖核酸水平升高。与在标准条件（20%氧气）下培养的人滋养层细胞系（BeWo细胞）相比，在低氧条件（5%氧气）下培养的该细胞系中瘦素分泌显著增加（P<0.01）。本研究表明，重度子痫前期患者胎盘瘦素的产生增加，可能是由于胎盘缺氧，从而提示瘦素作为重度子痫前期胎盘缺氧标志物的潜在重要性。
