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基质辅助激光解吸电离飞行时间质谱在鉴定苛养性儿科病原体 Aggregatibacter、Eikenella、嗜血杆菌和 Kingella 中的应用。

Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of the fastidious pediatric pathogens Aggregatibacter, Eikenella, Haemophilus, and Kingella.

机构信息

University of Cincinnati, Cincinnati, Ohio, USA.

出版信息

J Clin Microbiol. 2013 Nov;51(11):3862-4. doi: 10.1128/JCM.02233-13. Epub 2013 Aug 21.

DOI:10.1128/JCM.02233-13
PMID:23966506
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3889783/
Abstract

The accuracy of matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) in the identification of Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, and Kingella (HACEK) species was compared to that of phenotypic methods (Remel RapID and Vitek 2). Overall, Vitek MS correctly identified more isolates, incorrectly identified fewer isolates, and failed to identify fewer isolates than both phenotypic methods.

摘要

基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在鉴定嗜血杆菌、Aggregatibacter、心杆菌、爱肯菌和金氏菌（HACEK）物种方面的准确性与表型方法（Remel RapID 和 Vitek 2）进行了比较。总体而言，Vitek MS 正确鉴定的分离株更多，错误鉴定的分离株更少，未鉴定的分离株也比两种表型方法更少。

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本文引用的文献

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Rapid discrimination of Haemophilus influenzae, H. parainfluenzae, and H. haemolyticus by fluorescence in situ hybridization (FISH) and two matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) platforms.应用荧光原位杂交（FISH）和两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）平台快速鉴别流感嗜血杆菌、副流感嗜血杆菌和溶血性嗜血杆菌。
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Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification.两种基质辅助激光解吸电离飞行时间质谱系统用于细菌鉴定的稳健性。
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