[一种新型四小时诊断试剂盒——用于鉴定肠道细菌的RapID onE系统的评估]

[Assessment of a new four-hour diagnostic kit--RapID onE system for the identification of enteric bacteria].

作者信息

Lee J H, Huang M Z, Wu J L, Tsai W C

机构信息

National Yang-Ming University, Graduate Institute of Microbiology and Immunology, Taipei, Taiwan.

出版信息

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1994 Aug;27(3):133-9.

PMID:9747343

Abstract

RapID onE System is a newly developed four-hour rapid diagnostic kit for the identification of enteric bacteria. To know the effectiveness of this system, we used 125 strains of oxidase-negative, gram-negative bacilli for this evaluation. Except for Acinetobacter calcoaceticus, all the bacilli belong to family Enterobacteriaceae. The bacterial strains of this assessment belong to 12 genera and 20 species. Among them, 84 strains were freshly isolated from clinical specimens and 41 strains were frozen (-70 degrees C) stock clinical isolates. The results show that 115 (92.0%) strains were correctly identifed to the species level. It yielded 92.9% and 90.2% of correct identification of fresh isolates and frozen stocks, respectively. In this paper, the reading criteria of RapID onE System would also be discussed.

摘要

RapID onE系统是一种新开发的用于鉴定肠道细菌的四小时快速诊断试剂盒。为了解该系统的有效性，我们使用了125株氧化酶阴性的革兰氏阴性杆菌进行评估。除醋酸钙不动杆菌外，所有杆菌均属于肠杆菌科。本次评估的细菌菌株属于12个属和20个种。其中，84株是从临床标本中新鲜分离的，41株是冷冻（-70摄氏度）保存的临床分离株。结果显示，115株（92.0%）菌株被正确鉴定到种水平。新鲜分离株和冷冻保存株的正确鉴定率分别为92.9%和90.2%。本文还将讨论RapID onE系统的判读标准。