Kämpfer P
Fachgebiet Hygiene, Technischen Universität Berlin.
Zentralbl Bakteriol. 1990 Jun;273(2):164-72. doi: 10.1016/s0934-8840(11)80244-6.
The Titertek-Enterobac-Automated System (TTE-AS, Flow Laboratories, Meckenheim, FRG) was evaluated for identification of members of the family of Enterobacteriaceae using a total of 816 bacterial strains obtained from stock cultures and clinical specimens. A comparison of the results of the TTE-AS kit with conventional methods was conducted. Identifications were classified as correct, not identified (two or more taxa possible, identification score less than 80%, supplementary tests for final identification were not performed), and incorrect. Correct identification results were further subdivided according to the level of identification by species or biotype: (a) greater than or equal to 98%, (b) 90% to 97%, (c) 80% to 89%. When compared with conventional identification results, the TTE-AS system correctly identified 95.3% (778 of 816 strains), 90.0% (734 strains) belonging to category (a), 3.4% (28 strains) belonging to category (b), and 2.0% (16 strains) belonging to category (c). For 18 (2.2%) of the remaining strains, the system generated the correct identification result, but with an identification level less than 80% (low discrimination results). 11 strains (1.3%) were misidentified and the system failed to generate identification results with 9 strains (1.1%). Reactions within TTE-AS were reproducible with an estimated probability of erroneous test results of 1.7%.
使用从保藏培养物和临床标本中获得的总共816株细菌菌株，对Titertek-Enterobac自动系统（TTE-AS，德国美肯海姆弗洛实验室）进行了评估，以鉴定肠杆菌科成员。将TTE-AS试剂盒的结果与传统方法进行了比较。鉴定结果分为正确、未鉴定（可能有两个或更多分类单元，鉴定分数低于80%，未进行最终鉴定的补充试验）和错误。正确的鉴定结果根据按种或生物型的鉴定水平进一步细分：(a)大于或等于98%，(b)90%至97%，(c)80%至89%。与传统鉴定结果相比，TTE-AS系统正确鉴定了95.3%（816株中的778株），其中90.0%（734株）属于(a)类，3.4%（28株）属于(b)类，2.0%（16株）属于(c)类。对于其余18株（2.2%）菌株，该系统得出了正确的鉴定结果，但鉴定水平低于80%（鉴别结果低）。11株（1.3%）被误鉴定，该系统未能对9株（1.1%）菌株产生鉴定结果。TTE-AS内的反应具有可重复性，错误测试结果的估计概率为1.7%。
