Adam K P, Croteau R
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
Phytochemistry. 1998 Sep;49(2):475-80. doi: 10.1016/s0031-9422(97)00741-3.
(-)-Sabinene is the major monoterpene produced by a European strain of the liverwort Conocephalum conicum. A cell-free extract from in vitro cultured plants catalysed the cyclization of geranyl diphosphate to sabinene. The responsible monoterpene cyclase was partially purified and characterized as an operationally soluble enzyme of M(r) 65,000, with a pH optimum at 7.5 and a requirement for a divalent metal ion as the only cofactor, with Mg2+ preferred. The general properties of the sabinene synthase from C. conicum resemble those of other monoterpene cyclases from gymnosperms and angiosperms. A North American strain of the liverwort produces (+)-bornyl acetate as the major monoterpene and it was demonstrated that bornane-type monoterpenes are derived from geranyl diphosphate in this liverwort, as in higher plants, by the action of bornyl diphosphate synthase.
（-）-桧烯是欧洲地钱科植物角苔属的一种菌株产生的主要单萜。来自体外培养植物的无细胞提取物催化香叶基二磷酸环化生成桧烯。负责的单萜环化酶被部分纯化，其特征为一种分子量为65,000的可操作性溶解酶，最适pH为7.5，唯一的辅因子是二价金属离子，优先选择Mg2+。角苔属桧烯合酶的一般特性类似于裸子植物和被子植物的其他单萜环化酶。北美地钱科的一种菌株产生（+）-乙酸冰片酯作为主要单萜，并且已证明在该苔藓中，与高等植物一样，莰烷型单萜通过冰片基二磷酸合酶的作用源自香叶基二磷酸。
