Urwin R, Feavers I M, Jones D M, Maiden M C, Fox A J
Manchester Public Health Laboratory, Withington Hospital.
Epidemiol Infect. 1998 Aug;121(1):95-101. doi: 10.1017/s0950268898008942.
Changes in the frequency of serogroup B non serotypable (B:NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B:NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993-4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other 'serotype 4' isolates revealed five distinct porB alleles which were shown by nucleotide sequence determination to encode different peptide sequences. Differential binding of the 'serotype 4' mAbs MN14G21 and 5DC4C8G8 in whole cell ELISA and dot blot assays was the result, (i) of differences in the peptide sequence of predicted surface loop I and (ii) an amino acid deletion in predicted loop VI of the PorB protein.
通过T-track指纹分析、DNA核苷酸序列测定以及全细胞ELISA和斑点印迹法血清分型，对在英格兰和威尔士分离出的B群不可分型（B:NT）脑膜炎球菌的频率变化进行了调查。在1993 - 1994年期间，脑膜炎球菌参考单位（MRU）斑点印迹法指定为B:NT的分离株中，73%表达了血清分型抗原PorB的变体，通过全细胞ELISA检测为血清型4。这些以及其他“血清型4”分离株的T-track指纹图谱显示出五个不同的porB等位基因，通过核苷酸序列测定表明它们编码不同的肽序列。在全细胞ELISA和斑点印迹法中，“血清型4”单克隆抗体MN14G21和5DC4C8G8的差异结合是以下结果：（i）预测表面环I肽序列的差异，以及（ii）PorB蛋白预测环VI中的一个氨基酸缺失。
