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评估porB聚合酶链反应扩增产物限制性内切酶分析作为确定不可分型脑膜炎奈瑟菌中porB可变区序列的一种方法。

Evaluation of porB PCR-amplicon restriction endonuclease analysis as a method to determine porB variable-region sequences in nonserotypeable meningococci.

作者信息

Dyet Kristin H, Simmonds Robin S, Martin Diana R

机构信息

Communicable Disease Group, ESR Ltd., Porirua. Microbiology Department, University of Otago, Dunedin, New Zealand.

出版信息

J Clin Microbiol. 2004 Apr;42(4):1731-3. doi: 10.1128/JCM.42.4.1731-1733.2004.

DOI:10.1128/JCM.42.4.1731-1733.2004
PMID:15071034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387555/
Abstract

porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%).

摘要

孔蛋白B（PorB）聚合酶链反应（PCR）扩增片段限制性内切酶分析是一种快速、简便的方法，用于评估在新西兰脑膜炎球菌病流行期间分离出的不可分型脑膜炎球菌的PorB变异情况。在1990年至1999年期间（含1990年和1999年）分离出的大多数不可分型脑膜炎球菌要么属于4型（40.5%），要么含有PorB可变区1（VR1）-19、VR2-D、VR3-7和VR4-14a序列（45.1%）。

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本文引用的文献

1
Typing of Neisseria meningitidis isolates from patients with invasive disease by molecular analysis of porin genes.通过对孔蛋白基因进行分子分析对侵袭性疾病患者的脑膜炎奈瑟菌分离株进行分型。
New Microbiol. 2001 Apr;24(2):149-55.
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Molecular variation of meningococcal serotype 4 antigen genes.脑膜炎球菌4型血清型抗原基因的分子变异
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Correlation between serological and sequencing analyses of the PorB outer membrane protein in the Neisseria meningitidis serotyping system.脑膜炎奈瑟菌血清分型系统中PorB外膜蛋白的血清学分析与测序分析之间的相关性
Clin Diagn Lab Immunol. 1998 May;5(3):348-54. doi: 10.1128/CDLI.5.3.348-354.1998.
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New Zealand epidemic of meningococcal disease identified by a strain with phenotype B:4:P1.4.通过B:4:P1.4表型菌株确定的新西兰脑膜炎球菌病疫情。
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Typing of Neisseria meningitidis by restriction analysis of the amplified porA gene.通过对扩增的porA基因进行限制性分析对脑膜炎奈瑟菌进行分型。
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Characterization of Neisseria meningitidis by polymerase chain reaction and restriction endonuclease digestion of the porA gene.通过聚合酶链反应和对porA基因的限制性内切酶消化来鉴定脑膜炎奈瑟菌。
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Serotypes and subtypes of Neisseria meningitidis: results of an international study comparing sensitivities and specificities of monoclonal antibodies.脑膜炎奈瑟菌的血清型和亚型：一项比较单克隆抗体敏感性和特异性的国际研究结果
Clin Diagn Lab Immunol. 1995 Jan;2(1):69-72. doi: 10.1128/cdli.2.1.69-72.1995.
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Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China.通过分析编码1类外膜蛋白的基因中的限制性片段长度多态性对脑膜炎奈瑟菌进行分型：在中国过去40年中的疫情评估中的应用
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