评估porB聚合酶链反应扩增产物限制性内切酶分析作为确定不可分型脑膜炎奈瑟菌中porB可变区序列的一种方法。
Evaluation of porB PCR-amplicon restriction endonuclease analysis as a method to determine porB variable-region sequences in nonserotypeable meningococci.
作者信息
Dyet Kristin H, Simmonds Robin S, Martin Diana R
机构信息
Communicable Disease Group, ESR Ltd., Porirua. Microbiology Department, University of Otago, Dunedin, New Zealand.
出版信息
J Clin Microbiol. 2004 Apr;42(4):1731-3. doi: 10.1128/JCM.42.4.1731-1733.2004.
Abstract
porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%).
摘要
孔蛋白B（PorB）聚合酶链反应（PCR）扩增片段限制性内切酶分析是一种快速、简便的方法，用于评估在新西兰脑膜炎球菌病流行期间分离出的不可分型脑膜炎球菌的PorB变异情况。在1990年至1999年期间（含1990年和1999年）分离出的大多数不可分型脑膜炎球菌要么属于4型（40.5%），要么含有PorB可变区1（VR1）-19、VR2-D、VR3-7和VR4-14a序列（45.1%）。
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