Kang S G, Jin J B, Piao H L, Pih K T, Jang H J, Lim J H, Hwang I
Department of Molecular Biology, Gyeongsang National University, Chinju, Korea.
Plant Mol Biol. 1998 Oct;38(3):437-47. doi: 10.1023/a:1006099718761.
Dynamin-related proteins are high molecular weight GTPase proteins found in a variety of eukaryotic cells from yeast to human. They are involved in diverse biological processes that include endocytosis in animal cells and vacuolar protein sorting in yeast. We isolated a new gene, ADL2, that encodes a dynamin-like protein in Arabidopsis. The ADL2 cDNA is 2.68 kb in size and has an open reading frame for 809 amino acid residues with a calculated molecular mass of 90 kDa. Sequence analysis of ADL2 revealed a high degree of amino acid sequence similarity to other members of the dynamin superfamily. Among those members ADL2 was most closely related to Dnm1p of yeast and thus appears to be a member of the Vps1p subfamily. Expression studies showed that the ADL2 gene is widely expressed in various tissues with highest expression in flower tissues. In vivo targeting experiments showed that ADL2:smGFP fusion protein is localized to chloroplasts in soybean photoautroph cells. In addition experiments with deletion constructs revealed that the N-terminal 35 amino acid residues were sufficient to direct the smGFP into chloroplasts in tobacco protoplasts when expressed as a fusion protein.
动力蛋白相关蛋白是一类高分子量的GTP酶蛋白，存在于从酵母到人类的多种真核细胞中。它们参与多种生物学过程，包括动物细胞中的内吞作用和酵母中的液泡蛋白分选。我们从拟南芥中分离出一个新基因ADL2，它编码一种类似动力蛋白的蛋白。ADL2 cDNA大小为2.68 kb，有一个编码809个氨基酸残基的开放阅读框，计算分子量为90 kDa。ADL2的序列分析显示，其氨基酸序列与动力蛋白超家族的其他成员高度相似。在这些成员中，ADL2与酵母的Dnm1p关系最为密切，因此似乎是Vps1p亚家族的成员。表达研究表明，ADL2基因在各种组织中广泛表达，在花组织中表达最高。体内靶向实验表明，ADL2:smGFP融合蛋白定位于大豆光合自养细胞的叶绿体中。此外，缺失构建体的实验表明，当作为融合蛋白表达时，N端的35个氨基酸残基足以将smGFP导入烟草原生质体的叶绿体中。
