Takami K, Matsuo A, Terai K, Walker D G, McGeer E G, McGeer P L
Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, Canada.
Brain Res. 1998 Aug 17;802(1-2):89-97. doi: 10.1016/s0006-8993(98)00552-6.
Localization of fibroblast growth receptor (FGFR)-1 immunoreactivity was investigated immunochemically in postmortem brain tissue of Alzheimer's disease (AD) and age-matched control cases using a rabbit polyclonal antibody and a mouse monoclonal antibody specific for FGFR-1. In control cases, FGFR-1 immunoreactivity was identified in astrocytes in white matter and in hippocampal pyramidal neurons. In AD cases, the immunoreactivity in reactive astrocytes surrounding senile plaques was increased. The pattern of FGFR-1 immunoreactivity was confirmed in selected cases by in situ hybridization for FGFR-1 mRNA. Immunoreactivity using a monoclonal antibody demonstrated a similar distribution pattern. The localization of FGFR-1 is consistent with previous reports on the involvement of FGF-1 and FGF-2 in AD.
使用针对成纤维细胞生长受体（FGFR）-1的兔多克隆抗体和小鼠单克隆抗体，通过免疫化学方法研究了阿尔茨海默病（AD）患者和年龄匹配的对照病例的死后脑组织中FGFR-1免疫反应性的定位。在对照病例中，在白质中的星形胶质细胞和海马锥体细胞中鉴定出FGFR-1免疫反应性。在AD病例中，老年斑周围反应性星形胶质细胞中的免疫反应性增加。通过FGFR-1 mRNA原位杂交在选定病例中证实了FGFR-1免疫反应性模式。使用单克隆抗体的免疫反应性显示出相似的分布模式。FGFR-1的定位与先前关于FGF-1和FGF-2参与AD的报道一致。
