根癌土壤杆菌噬菌体DNA的物理特性

Physical characteristics of DNA from bacteriophages of Agrobacterium tumefaciens.

作者信息

Manasse R J, Staples R C

出版信息

Can J Microbiol. 1976 Oct;22(10):1583-5. doi: 10.1139/m76-232.

Abstract

DNA was extracted from isolates of bacteriophages grown on virulent and avirulent strains of Agrobacterium tumefaciens. Molecular weights of DNA from phages isolated from the virulent A. tumefaciens (IIBV7) were about 41.5 X 10(6) daltons, while those from the avirulent A. tumefaciens (IIBNV6) were about 32.5 X 10(6) daltons. The buoyant densities of the four DNA's ranged from 1.7086 to 1.7089 g/cm3, values that were not significantly different. DNA-DNA hybridization studies also indicated that the four phages were closely related. Attempts to induce tumors with phage DNA were unsuccessful.

摘要

从在根癌土壤杆菌的致病菌株和无毒菌株上生长的噬菌体分离物中提取DNA。从致病根癌土壤杆菌（IIBV7）分离的噬菌体的DNA分子量约为41.5×10⁶道尔顿，而从无毒根癌土壤杆菌（IIBNV6）分离的噬菌体的DNA分子量约为32.5×10⁶道尔顿。四种DNA的浮力密度在1.7086至1.7089 g/cm³范围内，这些值没有显著差异。DNA-DNA杂交研究也表明这四种噬菌体密切相关。用噬菌体DNA诱导肿瘤的尝试未成功。