Fiorentini C, Barbieri E, Falzano L, Matarrese P, Baffone W, Pianetti A, Katouli M, Kühn I, Möllby R, Bruscolini F, Casiere A, Donelli G
Department of Ultrastructures, Istituto Superiore di Sanita, Rome, Italy.
J Appl Microbiol. 1998 Sep;85(3):501-11. doi: 10.1046/j.1365-2672.1998.853517.x.
A total of 208 strains of Aeromonas were isolated by monthly sampling from two estuaries (one provided with, and the other devoid of a waste-water treatment system) on the Italian coast of the Adriatic sea between September 1994 and August 1995. Biotyping at the species level allowed the identification of 96 strains (46%) as Aer. caviae, 46 (22%) as Aer. sobria, 33 (16%) as Aer. hydrophila and 25 (12%) as Aer. veronii. Eight strains (4%) were regarded as unnamed aeromonads. Aeromonas caviae was the most prevalent species in water with a high degree of pollution, while Aer. hydrophila strains were more commonly isolated from cleaner water. Aeromonas sobria and Aer. veronii were equally distributed in both estuaries. There was no correlation between temperature and numbers of aeromonads in either estuary. Using a biochemical fingerprinting method, strains were divided into similarity groups (PhP-types) based on their biochemical phenotypes. Several different PhP-types were found in each estuary, yielding a high diversity for these strains. However, some identical PhP-types were also found in both estuaries and at different times of the year, indicating that certain Aeromonas strains can survive more widely varying physico-chemical conditions. The production of toxins capable of causing cytoskeletal-dependent changes in the morphology of Chinese hamster ovary (CHO) cells was detected in 14 strains and appeared to be dependent on the season.
1994年9月至1995年8月期间，通过每月采样，从亚得里亚海意大利海岸的两个河口（一个有废水处理系统，另一个没有）分离出总共208株气单胞菌。在种水平上进行生物分型，可鉴定出96株（46%）为豚鼠气单胞菌，46株（22%）为温和气单胞菌，33株（16%）为嗜水气单胞菌，25株（12%）为维罗纳气单胞菌。8株（4%）被视为未命名的气单胞菌。豚鼠气单胞菌是污染程度高的水中最常见的菌种，而嗜水气单胞菌菌株更常从较清洁的水中分离出来。温和气单胞菌和维罗纳气单胞菌在两个河口的分布相同。两个河口的温度与气单胞菌数量之间均无相关性。使用生化指纹图谱方法，根据菌株的生化表型将其分为相似组（PhP型）。在每个河口发现了几种不同的PhP型，这些菌株具有很高的多样性。然而，在两个河口以及一年中的不同时间也发现了一些相同的PhP型，这表明某些气单胞菌菌株能够在更广泛变化的理化条件下存活。在14株菌株中检测到能够引起中国仓鼠卵巢（CHO）细胞形态发生细胞骨架依赖性变化的毒素产生，并且似乎取决于季节。
