Noegel A, Gotschlich E C
J Exp Med. 1983 Jun 1;157(6):2049-60. doi: 10.1084/jem.157.6.2049.
Neisseria gonorrhoeae, as well as other Neisseriae, produce polyphosphate. This polyphosphate exists in two forms. Approximately half of it is loosely associated with the cells and can be recovered by washing in neutral buffers under conditions in which no significant lysis of the cells is observed. The other half is either intracellular or tightly associated, because it requires digestion of the cells with perchloric acid or sodium hypochlorite. Polyphosphate obtained by both methods was purified by column chromatography and chemically characterized. In contrast to other organisms, gonococci do not respond with increased polyphosphate synthesis when shifted from phosphate starvation to a phosphate-rich medium. In addition, gonococcal polyphosphate does not serve as a depletable phosphate source during phosphate starvation. All strains of Neisseriae examined produce substantial amounts of polyphosphate.
淋病奈瑟菌以及其他奈瑟菌属细菌会产生多聚磷酸盐。这种多聚磷酸盐以两种形式存在。大约一半与细胞松散结合，可通过在不观察到细胞明显裂解的条件下用中性缓冲液洗涤来回收。另一半要么存在于细胞内，要么紧密结合，因为它需要用高氯酸或次氯酸钠消化细胞才能获得。通过这两种方法获得的多聚磷酸盐经柱色谱纯化并进行了化学表征。与其他生物不同，淋病奈瑟菌从磷酸盐饥饿状态转变为富含磷酸盐的培养基时，多聚磷酸盐合成不会增加。此外，在磷酸盐饥饿期间，淋病奈瑟菌的多聚磷酸盐不作为可消耗的磷酸盐来源。所有检测的奈瑟菌属菌株都会产生大量的多聚磷酸盐。
