Petruzzelli S, Celi A, Pulerà N, Baliva F, Viegi G, Carrozzi L, Ciacchini G, Bottai M, Di Pede F, Paoletti P, Giuntini C
Cardiopulmonary Department, University of Pisa, Italy.
Cancer Res. 1998 Sep 15;58(18):4122-6.
Polycyclic aromatic hydrocarbons (PAHs) are almost ubiquitous pollutants that may interact with metabolic systems in human tissues and eventually cause cancer. PAH-adducted DNA becomes antigenic and antibodies anti-benzo(a)pyrene diol epoxide (BPDE)-DNA may be found in serum of PAH-exposed subjects. The presence of serum antibodies anti-BPDE-DNA adduct was investigated in 1345 individuals from family clusters of the general population of a small area in central Italy in whom information about smoking habits, site of residence, and personal and family history of lung diseases, including cancer, were obtained. Anti-BPDE-DNA antibodies in the sera were detected with a direct ELISA and the association of anti-BPDE-DNA antibodies with subjects' data from a standardized respiratory questionnaire including age, occupation, tobacco smoking habits, respiratory symptoms, and family history of respiratory diseases was subsequently tested by multivariate logistic regression analysis. The overall prevalence of subjects with anti-BPDE-DNA antibodies was 21.0% (n=283), with no differences between males and females. Anti-BPDE-DNA positivity was associated with living in the urban area [odds ratio (OR), 1.49; 95% confidence interval (CI), 1.16-1.92], with active tobacco smoking (OR, 1.25; 95% CI, 1.06-1.48), and with family history of lung cancer (OR, 1.30; 95% CI, 0.90-1.88), and positivity increased with the number of members in the family cluster positive to anti-BPDE-DNA antibodies (OR, 1.30; 95% CI, 1.03-1.65). This study on a large general population sample indicates that serum anti-BPDE-DNA antibodies may be considered as biomarkers of exposure to environmental carcinogens and of DNA damage. The genetic and familial components of their association with tobacco smoking lend further support to the argument about the familial predisposition to lung cancer.
多环芳烃（PAHs）是几乎无处不在的污染物，它们可能与人体组织中的代谢系统相互作用，最终导致癌症。PAH加合物DNA具有抗原性，在接触PAH的受试者血清中可能会发现抗苯并（a）芘二醇环氧化物（BPDE）-DNA抗体。在意大利中部一个小地区普通人群的家族群组中，对1345名个体进行了研究，收集了他们的吸烟习惯、居住地点以及包括癌症在内的肺部疾病个人和家族病史等信息，以调查血清中抗BPDE-DNA加合物抗体的存在情况。采用直接酶联免疫吸附测定法（ELISA）检测血清中的抗BPDE-DNA抗体，随后通过多变量逻辑回归分析检验抗BPDE-DNA抗体与来自标准化呼吸问卷的受试者数据之间的关联，这些数据包括年龄、职业、吸烟习惯、呼吸道症状以及呼吸道疾病家族史。抗BPDE-DNA抗体阳性受试者的总体患病率为21.0%（n = 283），男性和女性之间无差异。抗BPDE-DNA阳性与居住在城市地区相关[比值比（OR），1.49；95%置信区间（CI），1.16 - 1.92]，与当前吸烟相关（OR，1.25；95% CI，1.06 - 1.48），与肺癌家族史相关（OR，1.30；95% CI，0.90 - 1.88），并且随着家族群组中抗BPDE-DNA抗体阳性成员数量的增加，阳性率也升高（OR，1.30；95% CI，1.03 - 1.65）。这项针对大量普通人群样本的研究表明，血清抗BPDE-DNA抗体可被视为接触环境致癌物和DNA损伤的生物标志物。它们与吸烟之间关联的遗传和家族因素进一步支持了肺癌家族易感性的观点。
