Meier P, Müller U
Division of Internal Medicine, Zieglerspital, Bern, Switzerland.
Int Arch Allergy Immunol. 1998 Sep;117(1):46-51. doi: 10.1159/000023989.
Successful venom immunotherapy (VIT) in Hymenoptera allergy is usually associated with a strong increase in venom-specific serum IgG antibodies (sIgG).
We evaluated a new commercial test for the assay of sIgG (Pharmacia CAP Systemtrade mark IgG RAST(R) FEIA; FEIA), in comparison with a conventional ELISA technique. Sera from 40 bee- and 40 Vespula-allergic patients were analyzed by FEIA and ELISA before and 3 months after starting VIT.
The correlation between sIgG obtained with the two methods was significant: r = 0.862, p<0.0001 for bee venom (BV), r = 0.861, p<0.0001 for Vespula venom (VV). The geometric mean values obtained with FEIA were higher for both venoms (BV p = 0.03; VV p<0. 01). A highly significant increase (p<0.0001) was observed during VIT with both methods. This increase was concordant in 93% of VV- and 90% of BV-treated patients. Intra- and interassay relative coefficients of variation were below 10% for FEIA.
IgG RAST FEIA is a reproducible and sensitive method for the assay of venom-specific sIgG.
膜翅目过敏的成功毒液免疫疗法（VIT）通常与毒液特异性血清IgG抗体（sIgG）的显著增加有关。
我们评估了一种用于检测sIgG的新型商业检测方法（Pharmacia CAP System商标IgG RAST(R) FEIA；FEIA），并与传统ELISA技术进行比较。在开始VIT之前和之后3个月，通过FEIA和ELISA对40名蜜蜂过敏患者和40名黄蜂过敏患者的血清进行分析。
两种方法获得的sIgG之间的相关性显著：蜜蜂毒液（BV）的r = 0.862，p<0.0001；黄蜂毒液（VV）的r = 0.861，p<0.0001。两种毒液通过FEIA获得的几何平均值均更高（BV p = 0.03；VV p<0.01）。在VIT期间，两种方法均观察到高度显著的增加（p<0.0001）。在接受VV治疗的患者中，93%的患者增加情况一致；在接受BV治疗的患者中，90%的患者增加情况一致。FEIA的批内和批间相对变异系数均低于10%。
IgG RAST FEIA是一种用于检测毒液特异性sIgG的可重复且敏感的方法。
