Rittling S R, Feng F
Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA.
Biochem Biophys Res Commun. 1998 Sep 18;250(2):287-92. doi: 10.1006/bbrc.1998.9306.
Several different antibodies to mouse and/or rat osteopontin have been developed, and some antisera raised against human osteopontin have been shown to react with mouse osteopontin. We have taken advantage of the lack of osteopontin protein in mice with a targeted disruption of the osteopontin gene to characterize the reactivity and the specificity of several of these antibodies with mouse osteopontin by Western blotting. Our results demonstrate that, with the exception of the rat monoclonal antibody MPIIIB10, which does not recognize mouse osteopontin on Western blots, all the tested reagents do react with mouse osteopontin, but their sensitivity varies widely, and in some cases there is significant cross-reactivity of the antibodies with other proteins found in mouse tissue extracts.
已经开发出几种针对小鼠和/或大鼠骨桥蛋白的不同抗体，并且一些针对人骨桥蛋白产生的抗血清已被证明可与小鼠骨桥蛋白发生反应。我们利用骨桥蛋白基因靶向破坏的小鼠中缺乏骨桥蛋白蛋白这一特性，通过蛋白质印迹法来表征其中几种抗体与小鼠骨桥蛋白的反应性和特异性。我们的结果表明，除了大鼠单克隆抗体MPIIIB10（在蛋白质印迹中不识别小鼠骨桥蛋白）外，所有测试试剂均与小鼠骨桥蛋白发生反应，但其敏感性差异很大，并且在某些情况下，抗体与小鼠组织提取物中发现的其他蛋白质存在明显的交叉反应。
