Bodamyali T, Bhatt B, Hughes F J, Winrow V R, Kanczler J M, Simon B, Abbott J, Blake D R, Stevens C R
School of Postgraduate Medicine, University of Bath, Claverton Down, United Kingdom.
Biochem Biophys Res Commun. 1998 Sep 18;250(2):458-61. doi: 10.1006/bbrc.1998.9243.
Pulsed electromagnetic fields (PEMF) are successfully employed in the treatment of a variety of orthopaedic conditions, particularly delayed and nonunion fractures. In this study, we examined PEMF effects on in vitro osteogenesis by bone nodule formation and on mRNA expression of bone morphogenetic proteins 2 and 4 by reverse-transcriptase polymerase chain reaction (RT-PCR) in cultured rat calvarial osteoblasts. PEMF exposure induced a significant increase in both the number (39% over unexposed controls) and size (70% larger compared to unexposed controls) of bone-like nodules formed. PEMF also induced an increase in the levels of BMP-2 and BMP-4 mRNA in comparison to controls. This effect was directly related to the duration of PEMF exposure. This study shows that clinically applied PEMF have a reproducible osteogenic effect in vitro and simultaneously induce BMP-2 and -4 mRNA transcription. This supports the concept that the two effects are related.
脉冲电磁场（PEMF）已成功应用于多种骨科疾病的治疗，尤其是延迟愈合和不愈合骨折。在本研究中，我们通过骨结节形成检测了PEMF对体外成骨的影响，并通过逆转录聚合酶链反应（RT-PCR）检测了其对培养的大鼠颅骨成骨细胞中骨形态发生蛋白2和4的mRNA表达的影响。PEMF暴露导致形成的类骨结节数量（比未暴露对照组增加39%）和大小（比未暴露对照组大70%）均显著增加。与对照组相比，PEMF还诱导了BMP-2和BMP-4 mRNA水平的升高。这种效应与PEMF暴露的持续时间直接相关。本研究表明，临床应用的PEMF在体外具有可重复的成骨作用，并同时诱导BMP-2和-4 mRNA转录。这支持了这两种效应相关的概念。
