Lefèvre G, Beljean-Leymarie M, Beyerle F, Bonnefont-Rousselot D, Cristol J P, Thérond P, Torreilles J
Service de biochimie, Hôpital Tenon, Paris.
Ann Biol Clin (Paris). 1998 May-Jun;56(3):305-19.
Malondialdehyde assay is the most generally used test in the appreciation of the role of oxidative stress in disease. Malondialdehyde is one of several products formed during the radical induced decomposition of polyunsaturated fatty acids. Most often, malondialdehyde assay used its reactivity at high temperature and low pH, towards thiobarbituric acid. This reaction is very sensitive but its specificity, even with improvement of pre-analytical (sampling, preservatives), and analytical stages (fluorescence, HPLC) is still a matter of debate. At present, the concept of "thiobarbituric acid reactive substances" (TBARS) have merged and progressively replaced the initial malondialdehyde assay. In this review, we presented the main results concerning the assays of TBARS and malondialdehyde in blood and different biological medium. In the future, oxidative stress appreciation will need the precise analytical determination of different molecules triggered by free radicals. The TBARS assay should be considered as a global test, allowing a global approach of lipoperoxidation whereas specific determination of malondialdehyde can only appreciate one of the end-product formed during oxidative stress.
丙二醛检测是评估氧化应激在疾病中作用时最常用的检测方法。丙二醛是多不饱和脂肪酸自由基诱导分解过程中形成的几种产物之一。丙二醛检测通常利用其在高温和低pH条件下与硫代巴比妥酸的反应活性。该反应非常灵敏，但其特异性，即使在分析前阶段（采样、防腐剂）和分析阶段（荧光、高效液相色谱）有所改进后，仍存在争议。目前，“硫代巴比妥酸反应性物质”（TBARS）的概念已经合并，并逐渐取代了最初的丙二醛检测。在本综述中，我们介绍了血液和不同生物介质中TBARS和丙二醛检测的主要结果。未来，氧化应激评估将需要对自由基引发的不同分子进行精确的分析测定。TBARS检测应被视为一种全面检测，可对脂质过氧化进行全面评估，而丙二醛的特异性测定只能评估氧化应激过程中形成的一种终产物。
