El-Shafey Rabab Shaban, Baloza Samar H, Mohammed Lina Abdelhady, Nasr Hend Elsayed, Soliman Mohamed Mohamed, Ghamry Heba I, Elgendy Salwa A
Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Benha University, Benha 13511, Egypt.
Genetic and Genetic Engineering, Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt.
Toxicol Res (Camb). 2022 Mar 31;11(2):325-338. doi: 10.1093/toxres/tfac012. eCollection 2022 Apr.
This study examined if wheat germ oil (WGO) has gastroprotective impacts against ethanol-induced gastric ulcer in rats. Rats were assigned into control, WGO, ethanol, omeprazole + ethanol, and WGO + ethanol. WGO prevented gastric ulceration and damage induced by ethanol, the same effect induced by omeprazole, a widely known medication used for gastric ulcer treatment. WGO reduced gastric ulcer index, nitric oxide, and malondialdehyde levels in the stomach. WGO boosted the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), Bcl2, and the antioxidants. WGO showed inflammatory and anti-inflammatory impacts through the control of interleukin (IL)-1β, Tumor necrosis factor alpha (TNF-α), and IL-10 that were altered in ethanol-administered rats. Ethanol up-regulated caspase-3 and nuclear factor-kappa B (NF-kB) expression and showed histopathological changes such as necrosis and mucosal degeneration that were mitigated by pre-administration of WGO. Moreover, WGO decreased gastric immunoreactivity of NF-kB and increased transforming growth factor beta-1 (TGF-β1) that were associated with upregulation of Nrf2, heme oxygenase-1 (HO-1), and antioxidant expression and production. In conclusion, WGO reduced ethanol-induced stomach toxicity by regulating genes involved in oxidative stress, inflammation, and apoptotic/antiapoptotic pathways.
本研究检测了小麦胚芽油（WGO）对大鼠乙醇诱导型胃溃疡是否具有胃保护作用。将大鼠分为对照组、WGO组、乙醇组、奥美拉唑+乙醇组和WGO+乙醇组。WGO预防了乙醇诱导的胃溃疡和损伤，其效果与奥美拉唑相同，奥美拉唑是一种广为人知的用于治疗胃溃疡的药物。WGO降低了胃中的胃溃疡指数、一氧化氮和丙二醛水平。WGO促进了核因子红细胞2相关因子2（Nrf2）、血红素加氧酶-1（HO-1）、Bcl2和抗氧化剂的表达。WGO通过控制白细胞介素（IL）-1β、肿瘤坏死因子α（TNF-α）和IL-10发挥炎症和抗炎作用，这些因子在给予乙醇的大鼠中发生了改变。乙醇上调了半胱天冬酶-3和核因子-κB（NF-κB）的表达，并显示出组织病理学变化，如坏死和黏膜变性，而预先给予WGO可减轻这些变化。此外，WGO降低了NF-κB的胃免疫反应性，并增加了转化生长因子β-1（TGF-β1），这与Nrf2、血红素加氧酶-1（HO-1）以及抗氧化剂表达和产生的上调有关。总之，WGO通过调节参与氧化应激、炎症和凋亡/抗凋亡途径的基因，降低了乙醇诱导的胃毒性。
