Ushiki T, Abe K
Department of Anatomy, Niigata University School of Medicine, Japan.
Arch Histol Cytol. 1998 Aug;61(3):215-9. doi: 10.1679/aohc.61.215.
The present study describes a method for discriminating between the arterial and venous segments of blood vessels in mouse tissues and organs using alkaline phosphatase (ALPase) staining of ink/gelatin injected tissues. Anesthetized mice were injected through the left ventricle with blue ink/gelatin, and various organs and tissues were removed from the body and fixed by immersion in 10% formalin. Sections 50-100 microm thick were incubated for ALPase in a medium containing naphthol AS-BI phosphate and fast red TR by the azocoupling method. In such specimens as the brain and skeletal muscles, ALPase activity was found in arterioles and capillaries on the arterial side, whereas it was absent in capillaries on the venous side and in venules. In the liver, only branches of the hepatic artery were positive. ALPase activity was absent in the vessels of the lung except for a positive reaction in branches of the bronchial arteries. These findings indicate that the ALPase activity is confined to the arterioles and arterial segments of the capillaries in the systemic circuit. Thus, ALPase staining of ink/gelatin injected specimens is a useful method for differentiating the arterial and venous segments of the micro-vascular bed in various organs and tissues in mice.
本研究描述了一种通过对注射墨水/明胶的小鼠组织和器官进行碱性磷酸酶（ALPase）染色来区分血管动脉段和静脉段的方法。将麻醉的小鼠通过左心室注射蓝色墨水/明胶，然后从体内取出各种器官和组织，浸入10%福尔马林中固定。采用偶氮偶联法，将50-100微米厚的切片在含有萘酚AS-BI磷酸酯和固红TR的培养基中进行ALPase孵育。在脑和骨骼肌等标本中，动脉侧的小动脉和毛细血管中发现有ALPase活性，而静脉侧的毛细血管和小静脉中则没有。在肝脏中，只有肝动脉分支呈阳性。除支气管动脉分支有阳性反应外，肺血管中无ALPase活性。这些发现表明，ALPase活性局限于体循环中小动脉和毛细血管的动脉段。因此，对注射墨水/明胶的标本进行ALPase染色是区分小鼠各种器官和组织中微血管床动脉段和静脉段的一种有用方法。
