Taninishi Hideki, Jung Jin Yong, Izutsu Miwa, Wang Zhengfeng, Sheng Huaxin, Warner David S
Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, NC, United States; Department of Anesthesiology, Duke University Medical Center, Durham, NC, United States.
Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, NC, United States; Department of Surgery, Duke University Medical Center, Durham, NC, United States.
J Neurosci Methods. 2015 Feb 15;241:111-20. doi: 10.1016/j.jneumeth.2014.12.006. Epub 2014 Dec 17.
Laser Doppler flowmetry (LDF) is widely used for estimating cerebral blood flow changes during intraluminal middle cerebral artery occlusion (MCAO). No investigation has systematically examined LDF efficacy in standardizing outcome. We examined MCAO histologic and behavioral outcome as a function of LDF measurement.
Rats were subjected to 90min MCAO by 4 surgeons having different levels of MCAO surgical experience. LDF was measured in all rats during ischemia. By random assignment, LDF values were (Assisted) or were not (Blinded) made available to each surgeon during MCAO (n=12-17 per group). Neurologic and histologic outcomes were measured 7 days post-MCAO. A second study examined LDF effects on 1-day post-MCAO outcome.
Pooled across surgeons, intra-ischemic %LDF change (P=0.12), neurologic scores (Assisted vs. Blinded=14±6 vs. 13±7, P=0.61, mean±standard deviation) and cerebral infarct volume (162±63mm(3)vs. 143±86mm(3), P=0.24) were not different between groups. Only for one surgeon (novice) did LDF use alter infarct volume (145±28mm(3)vs. 98±61mm(3), P=0.03). LDF use decreased infarct volume coefficient of variation (COV) by 35% (P=0.02), but had no effect on neurologic score COV.
We compared intraluminal MCAO outcome as a function of LDF use.
LDF measurement altered neither neurologic nor histologic MCAO outcome. LDF did not decrease neurologic deficit COV, but did decrease infarct volume COV. LDF may allow use of fewer animals if infarct volume is the primary dependent variable, but is unlikely to impact requisite sample sizes if neurologic function is of primary interest.
激光多普勒血流仪（LDF）被广泛用于评估大脑中动脉腔内闭塞（MCAO）期间的脑血流变化。尚无研究系统地检验LDF在标准化结果方面的有效性。我们研究了作为LDF测量函数的MCAO组织学和行为学结果。
由4位具有不同MCAO手术经验水平的外科医生对大鼠进行90分钟的MCAO手术。在缺血期间对所有大鼠进行LDF测量。通过随机分配，在MCAO期间将LDF值提供给（辅助组）或不提供给（盲法组）每位外科医生（每组n = 12 - 17）。在MCAO后7天测量神经学和组织学结果。第二项研究检查了LDF对MCAO后1天结果的影响。
汇总所有外科医生的数据，缺血期间LDF变化百分比（P = 0.12）、神经学评分（辅助组与盲法组分别为14±6与13±7，P = 0.61，平均值±标准差）和脑梗死体积（162±63mm³对143±86mm³，P = 0.24）在两组之间无差异。仅对于一位外科医生（新手），使用LDF改变了梗死体积（145±28mm³对98±61mm³，P = 0.03）。使用LDF使梗死体积变异系数（COV）降低了35%（P = 0.02），但对神经学评分COV无影响。
我们比较了作为LDF使用函数的腔内MCAO结果。
LDF测量既未改变MCAO的神经学结果也未改变组织学结果。LDF未降低神经功能缺损COV，但降低了梗死体积COV。如果梗死体积是主要因变量，LDF可能允许使用更少的动物，但如果主要关注神经功能，则不太可能影响所需的样本量。
