Swiergosz R, Zakrzewska M, Sawicka-Kapusta K, Bacia K, Janowska I
Department of Animal Ecology, Jagiellonian University, Ingardena 6, Krakow, 30-060, Poland.
Ecotoxicol Environ Saf. 1998 Oct;41(2):130-6. doi: 10.1006/eesa.1998.1677.
Cadmium is one of many metals that are not physiologically or biochemically essential to organisms. This element is extremely dangerous as it is easily absorbed and remains in tissues for a long time. Long exposure to high doses of cadmium may cause biochemical and functional changes in some critical organs. In this study, wheat grains contaminated with cadmium chloride were used to test the influence of cadmium on male bank voles (Clethrionomys glareolus). Doses used in the experiment were environmentally realistic: 0.25 microg g-1 (control), 15 microg g-1, and 40 microg g-1 cadmium (dry weight). The animals were given cadmium-contaminated food and clean water ad libitum for 3 and 6 months. After these exposures, the animals were killed and the kidneys, liver, and testes from each vole were collected for analyses. The concentrations of Cd, Cu, Zn, and Fe in the tissues were determined with an atomic absorption spectrophotometer. The formalin-fixed testes, kidneys, and part of the liver were embedded in paraffin and then stained with hematoxylin and eosin. Cadmium accumulation in the tissues was directly proportional to dose. The highest cadmium concentrations were found in the kidneys of animals fed the highest dose of cadmium. Histological examination of the tissues revealed some pathological changes in the structure of kidneys, liver, and testes.
镉是众多对生物体没有生理或生化必要性的金属之一。这种元素极其危险，因为它很容易被吸收并长时间留存于组织中。长期暴露于高剂量的镉可能会导致一些关键器官发生生化和功能变化。在本研究中，用被氯化镉污染的麦粒来测试镉对雄性小林姬鼠（Clethrionomys glareolus）的影响。实验中使用的剂量符合环境实际情况：0.25微克/克（对照）、15微克/克和40微克/克镉（干重）。给动物随意提供受镉污染的食物和清洁水，持续3个月和6个月。在这些暴露期过后，将动物处死，并收集每只小林姬鼠的肾脏、肝脏和睾丸用于分析。用原子吸收分光光度计测定组织中镉、铜、锌和铁的浓度。将用福尔马林固定的睾丸、肾脏和部分肝脏包埋在石蜡中，然后用苏木精和伊红染色。组织中的镉积累与剂量成正比。在喂食最高剂量镉的动物的肾脏中发现了最高的镉浓度。对组织的组织学检查揭示了肾脏、肝脏和睾丸结构中的一些病理变化。
