Kindermann J, El-Ayouti Y, Samuels GJ, Kubicek CP
Abteilung fur Mikrobielle Biochemie, Institut fur Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9-172.5, Wien, A-1060, Austria.
Fungal Genet Biol. 1998 Aug;24(3):298-309. doi: 10.1006/fgbi.1998.1049.
Sequences of the internal transcribed spacer region 1 (ITS1) of the ribosomal DNA were used to determine the phylogenetic relationships of species of Trichoderma sect. Pachybasium. To this end, 85 strains-including all the available ex-type strains-were analyzed. Parsimony analysis demonstrated that the section is nonmonophyletic, distributing the 85 strains among three main groups that were supported by bootstrap values. Group A comprises two clades (A1 and A2), with A1 including T. polysporum, T. piluliferum, and T. minutisporum, while A2 included T. hamatum, T. pubescens, and T. strigosum in addition to species previously included in sect. Trichoderma (i.e., T. viride, T. atroviride, and T. koningii). The ex-type strain of T. fasciculatum formed a separate branch basal to clade A. Clade B contained the sect. Pachybasium members T. harzianum, T. fertile, T. croceum, T. longipile, T. strictipile, T. tomentosum, T. oblongisporum, T. flavofuscum, T. spirale, and the anamorphs of Hypocrea semiorbis and H. cf. gelatinosa. Sequence differences among clades A1, A2, and B were in the same order of magnitude as between each of them and T. longibrachiatum, which was used as an outgroup in these analyses. Sequence differences within clades A1, A2, and B were considerably smaller: in some cases (i.e., T. virens and T. flavofuscum; T. strictipile and H. cf. gelatinosa), the ITS1-sequences were identical, suggesting conspecifity. In other cases (e.g., T. crassum and T. longipile; T. harzianum, T. inhamatum, T. croceum, T. fertile, and H. semiorbis; T. hamatum and T. pubescens; and T. viride, T. atroviride, and T. koningii) differences were in the range of 1-3 nt only, suggesting a very close phylogenetic relationship. The sequence of a previously described aggressive mushroom competitor group of T. harzianum strains (Th2) was strikingly different from that of the ex-type strain of T. harzianum and closely related species and is likely to be a separate species. Copyright 1998 Academic Press.
核糖体DNA内转录间隔区1（ITS1）序列被用于确定木霉属厚基组物种间的系统发育关系。为此，对85个菌株进行了分析，其中包括所有可用的模式菌株。简约分析表明，该组并非单系类群，85个菌株分布在三个主要类群中，这些类群得到了自展值的支持。A组包含两个分支（A1和A2），A1包括多孢木霉、丸形木霉和微小孢木霉，而A2除了厚基组中先前包含的物种（即绿色木霉、深绿木霉和康氏木霉）外，还包括钩状木霉、柔毛木霉和糙皮木霉。束状木霉的模式菌株在A分支基部形成一个单独的分支。B分支包含厚基组成员哈茨木霉、可育木霉、橘黄木霉、长梗木霉、硬梗木霉、绒毛木霉、长孢木霉、黄褐木霉、螺旋木霉，以及半轮生肉座菌和类明胶肉座菌的无性型。A1、A2和B分支之间的序列差异与它们各自与长枝木霉（在这些分析中用作外类群）之间的差异处于同一数量级。A1、A2和B分支内的序列差异要小得多：在某些情况下（即绿色木霉和黄褐木霉；硬梗木霉和类明胶肉座菌），ITS1序列相同，表明为同种。在其他情况下（如粗木霉和长梗木霉；哈茨木霉、内哈茨木霉、橘黄木霉、可育木霉和半轮生肉座菌；钩状木霉和柔毛木霉；以及绿色木霉、深绿木霉和康氏木霉）差异仅在1至3个核苷酸范围内，表明系统发育关系非常密切。先前描述的哈茨木霉菌株的一个具有侵略性的蘑菇竞争群体（Th2）的序列与哈茨木霉模式菌株及相关物种的序列明显不同，很可能是一个单独的物种。版权所有1998年学术出版社。
