Hofmann M, Bossy S
Institut für Viruskrankheiten und Immunprophylaxe (IVI), Mittelhäusern.
Schweiz Arch Tierheilkd. 1998;140(9):365-70.
RT-PCR followed by direct nucleotide sequencing of the amplified cDNA was carried out to analyse most of the 5' nontranslated region (5'NTR) of classical swine fever virus (CSFV) isolates from the five 1993 disease outbreaks in Switzerland. Sequence data were compared to other CSFV strains, and dendrograms were constructed in order to determine the phylogenetic relationship of the Swiss virus strains. Dendrograms formed by the analysis of different parts of the 5'NTR were compared. It was shown that all Swiss isolates were related to other CSFV strains involved in disease outbreaks in Europe in the 1990s. Two of the isolates were indistinguishable from a CSFV strain isolated from wild boar meat imported from China into Austria in 1993. The risk of introducing classical swine fever by improperly treated swill and, in particular by importing wild boar meat is discussed.
采用逆转录聚合酶链反应(RT-PCR),随后对扩增的互补脱氧核糖核酸(cDNA)进行直接核苷酸测序,以分析来自瑞士1993年五次疾病暴发的经典猪瘟病毒(CSFV)分离株的大部分5'非翻译区(5'NTR)。将序列数据与其他CSFV毒株进行比较,并构建系统发育树以确定瑞士病毒株的系统发育关系。比较了通过分析5'NTR不同部分形成的系统发育树。结果表明,所有瑞士分离株均与20世纪90年代欧洲疾病暴发中涉及的其他CSFV毒株有关。其中两个分离株与1993年从中国进口到奥地利的野猪肉中分离出的一株CSFV毒株无法区分。讨论了因泔水处理不当,特别是进口野猪肉而引入经典猪瘟的风险。