Chédin F, Seitz E M, Kowalczykowski S C
Section of Microbiology, University of California, Davis 95616-8665, USA.
Trends Biochem Sci. 1998 Aug;23(8):273-7. doi: 10.1016/s0968-0004(98)01243-2.
In Bacteria and Eukarya, ssDNA-binding proteins are central to most aspects of DNA metabolism. Until recently, however, no counterpart of an ssDNA-binding protein had been identified in the third domain of life, Archaea. Here, we report the discovery of a novel type of ssDNA-binding protein in the genomes of several archaeons. These proteins, in contrast to all known members of this protein family, possess four conserved DNA-binding sites within a single polypeptide or, in one case, two polypeptides. This peculiar structural organization allows us to propose a model for the evolution of this class of proteins.
在细菌和真核生物中，单链DNA结合蛋白是DNA代谢大多数方面的核心。然而，直到最近，在生命的第三个域——古菌中，尚未发现单链DNA结合蛋白的对应物。在此，我们报告在几种古菌的基因组中发现了一种新型单链DNA结合蛋白。与该蛋白家族的所有已知成员不同，这些蛋白在单个多肽内或在一种情况下在两个多肽内具有四个保守的DNA结合位点。这种独特的结构组织使我们能够提出这类蛋白的进化模型。
