Lamosa P, Martins LO, Santos H
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 2780 Oeiras, Portugal.
Appl Environ Microbiol. 1998 Oct;64(10):3591-8. doi: 10.1128/AEM.64.10.3591-3598.1998.
The effects of salinity and growth temperature on the accumulation of intracellular organic solutes were examined by nuclear magnetic resonance spectroscopy (NMR) in Thermococcus litoralis, Thermococcus celer, Thermococcus stetteri, and Thermococcus zilligii (strain AN1). In addition, the effects of growth stage and composition of the medium were studied in T. litoralis. A novel compound identified as beta-galactopyranosyl-5-hydroxylysine was detected in T. litoralis grown on peptone-containing medium. Besides this newly discovered compound, T. litoralis accumulated mannosylglycerate, aspartate, alpha-glutamate, di-myo-inositol-1,1'(3,3')-phosphate, hydroxyproline, and trehalose. The hydroxyproline and beta-galactopyranosyl-5-hydroxylysine were probably derived from peptone, while the trehalose was derived from yeast extract; none of these three compounds was detected in the other Thermococcus strains examined. Di-myo-inositol-1,1'(3,3')-phosphate, aspartate, and mannosylglycerate were detected in T. celer and T. stetteri, and the latter organism also accumulated alpha-glutamate. The only nonmarine species studied, T. zilligii, accumulated very low levels of alpha-glutamate and aspartate. The levels of mannosylglycerate and aspartate increased in T. litoralis, T. celer, and T. stetteri in response to salt stress, while di-myo-inositol-1,1'(3,3')-phosphate was the major intracellular solute at supraoptimal growth temperatures. The phase of growth had a strong influence on the types and levels of compatible solutes in T. litoralis; mannosylglycerate and aspartate were the major solutes during exponential growth, while di-myo-inositol-1,1'(3,3')-phosphate was the predominant organic solute during the stationary phase of growth. This work revealed an unexpected ability of T. litoralis to scavenge suitable components from the medium and to use them as compatible solutes.
利用核磁共振光谱（NMR）研究了盐度和生长温度对嗜热栖热菌、嗜热栖热栖热菌、嗜热栖热栖热菌和嗜热栖热栖热菌（菌株AN1）细胞内有机溶质积累的影响。此外，还研究了嗜热栖热菌的生长阶段和培养基组成的影响。在以含蛋白胨培养基培养的嗜热栖热菌中检测到一种新化合物，鉴定为β-吡喃半乳糖基-5-羟基赖氨酸。除了这种新发现的化合物外，嗜热栖热菌还积累了甘露糖甘油酸、天冬氨酸、α-谷氨酸、二-肌醇-1,1'（3,3'）-磷酸、羟脯氨酸和海藻糖。羟脯氨酸和β-吡喃半乳糖基-5-羟基赖氨酸可能来源于蛋白胨，而海藻糖来源于酵母提取物；在所检测的其他嗜热栖热菌菌株中均未检测到这三种化合物。在嗜热栖热栖热菌和嗜热栖热栖热菌中检测到二-肌醇-1,1'（3,3'）-磷酸、天冬氨酸和甘露糖甘油酸，后者还积累了α-谷氨酸。所研究的唯一非海洋物种嗜热栖热栖热菌积累的α-谷氨酸和天冬氨酸水平非常低。在嗜热栖热菌、嗜热栖热栖热菌和嗜热栖热栖热菌中，甘露糖甘油酸和天冬氨酸的水平因盐胁迫而增加，而二-肌醇-1,1'（3,3'）-磷酸是超最适生长温度下的主要细胞内溶质。生长阶段对嗜热栖热菌中相容性溶质的类型和水平有很大影响；甘露糖甘油酸和天冬氨酸在指数生长期间是主要溶质，而二-肌醇-1,1'（3,3'）-磷酸是生长稳定期的主要有机溶质。这项工作揭示了嗜热栖热菌具有从培养基中清除合适成分并将其用作相容性溶质的意外能力。
