Schmid J, Hämmerle C H, Flückiger L, Winkler J R, Olah A J, Gogolewski S, Lang N P
Department of Periodontology and Fixed Prosthodontics, University of Berne, Switzerland.
Clin Oral Implants Res. 1997 Apr;8(2):75-81. doi: 10.1034/j.1600-0501.1997.080201.x.
The aim of the present study was to evaluate the effect of natural deproteinized bone mineral on the temporal and spatial pattern of bone formation in a guided bone regeneration model system while using a bioresorbable membrane device. A periosteal skin flap was raised uncovering the calvaria of 20 rabbits. A stiff hemispherical dome made of polylactic acid was placed onto the roughened calvaria and anchored by screws. Prior to placement, the dome was either filled with peripheral blood (control group, 8 rabbits) or with blood and OsteoGraf/N-300 (test group, 12 rabbits). At 1 month, histologic sections revealed bone regeneration in both test and control domes to various degrees. In the test domes, bone height reached 78% (67-83) and bone volume was 11% (6-17), while in the control domes, bone height was 45% (14-67) and bone volume 6% (1-11). At 2 months, bone height was unchanged in the test group at 70% (67-83) and bone volume had only slightly increased to 16% (11-21). In the controls, height increased to 86% (60-100) and volume to 20% (9-27). Thus, in this model system, natural bone mineral fill contributed to accelerate initial bone neogenesis, while it did not contribute to increasing bone volume or bone height at later observation stages.
本研究的目的是在使用生物可吸收膜装置的引导性骨再生模型系统中，评估天然脱蛋白骨矿物质对骨形成的时间和空间模式的影响。掀起骨膜皮瓣，暴露20只兔子的颅骨。将由聚乳酸制成的坚硬半球形穹顶放置在粗糙的颅骨上，并用螺钉固定。在放置前，穹顶要么填充外周血（对照组，8只兔子），要么填充血液和OsteoGraf/N - 300（试验组，12只兔子）。1个月时，组织学切片显示试验组和对照组的穹顶均有不同程度的骨再生。在试验组穹顶中，骨高度达到78%（67 - 83），骨体积为11%（6 - 17），而在对照组穹顶中，骨高度为45%（14 - 67），骨体积为6%（1 - 11）。2个月时，试验组的骨高度保持在70%（67 - 83）不变，骨体积仅略有增加至16%（11 - 21）。在对照组中，高度增加到86%（60 - 100），体积增加到20%（9 - 27）。因此，在该模型系统中，天然骨矿物质填充有助于加速早期骨新生，但在后期观察阶段对增加骨体积或骨高度并无作用。
