Zhang Y, Danielsen N, Sundler F, Mulder H
Department of Physiology and Neuroscience, University of Lund, Lund University Hospital, Sweden.
Neuroreport. 1998 Aug 24;9(12):2833-6. doi: 10.1097/00001756-199808240-00027.
The neuropeptide pituitary adenylate cyclase-activating peptide (PACAP) is expressed in sensory neurons. Expression of several neuropeptides is up-regulated in sensory neurons following inflammation. To examine whether also PACAP expression is regulated by inflammation, PACAP expression in L5 dorsal root ganglion (DRG) was determined, using in situ hybridization, after unilateral adjuvant-induced inflammation in the rat paw. At 12 h and day 3, but not day 21, the percentage of neurons expressing PACAP mRNA was greater in the innervating L5 DRG. Similarly, PACAP mRNA expression in individual neurons was higher in the innervating L5 DRG at 12 h and day 3, but not day 21. Up-regulated PACAP expression following adjuvant injection suggests a role for PACAP in inflammation.
神经肽垂体腺苷酸环化酶激活肽（PACAP）在感觉神经元中表达。炎症后，几种神经肽在感觉神经元中的表达会上调。为了研究PACAP的表达是否也受炎症调节，在大鼠爪部单侧注射佐剂诱导炎症后，采用原位杂交法测定了L5背根神经节（DRG）中PACAP的表达。在12小时和第3天，而非第21天，支配L5 DRG中表达PACAP mRNA的神经元百分比更高。同样，在12小时和第3天，而非第21天，支配L5 DRG中单个神经元的PACAP mRNA表达更高。佐剂注射后PACAP表达上调表明PACAP在炎症中发挥作用。
