Jánosi I M, Chrétien D, Flyvbjerg H
Höchstleistungsrechenzentrum (HRLZ), Forschungszentrum Jülich, Germany.
Eur Biophys J. 1998;27(5):501-13. doi: 10.1007/s002490050160.
Electron micrographs of tips of growing and shrinking microtubules are analyzed and interpreted. The many shapes observed are all consistent with a simple mechanical model, a flexible tube with competing intrinsic curvatures. Observations are also consistent with growing and shrinking microtubules having the same intrinsic curvature for protofilaments, the one observed in oligomers peeling off shrinking microtubules. If this is so, the lateral bonds between protofilaments are responsible for the difference between shapes of tips on growing and shrinking microtubules.
对正在生长和收缩的微管尖端的电子显微照片进行了分析和解读。观察到的多种形状均与一个简单的力学模型相符，即一个具有相互竞争的固有曲率的柔性管。观察结果还表明，正在生长和收缩的微管对于原纤维具有相同的固有曲率，这一固有曲率与从收缩微管上剥离的寡聚体中观察到的一致。如果真是这样，那么原纤维之间的横向键就决定了生长和收缩微管尖端形状的差异。
