Deraz Sahar F, Karlsson Eva Nordberg, Khalil Ashraf A, Mattiasson Bo
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, 22100 Lund, Sweden.
J Ind Microbiol Biotechnol. 2007 May;34(5):373-9. doi: 10.1007/s10295-007-0206-8. Epub 2007 Jan 26.
Lactobacillus acidophilus DSM 20079 is the producer of a novel bacteriocin termed acidocin D20079. In this paper, mode of action using three various concentrations of acidocin D20079 (2,048, 128 and 11.3 AU/ml) was determined against an indicator strain L. delbrueckii subsp. lactis DSM 20076. These concentrations all led to marked decreases in both the number of viable cells and in optical density, indicating that the activity of the acidocin D20079 was bactericidal with concomitant cell lysis. Moreover, the probiotic potential of L. acidophilus DSM 20079 was analyzed for its ability to survive and retain viability at conditions (acid and bile concentrations) mimicking the gastrointestinal (GI) tract, under which it survived exposure to pH 2.0 with a 1.2 log cycle reduction in viability and where 45% of the original population survived in a medium containing 0.3% bile for 3 h.
嗜酸乳杆菌DSM 20079是一种新型细菌素——嗜酸菌素D20079的产生菌。在本文中，使用三种不同浓度（2048、128和11.3 AU/ml）的嗜酸菌素D2007针对指示菌株德氏乳杆菌乳酸亚种DSM 20076测定了其作用方式。这些浓度均导致活菌数和光密度显著下降，表明嗜酸菌素D20079的活性具有杀菌作用并伴有细胞裂解。此外，还分析了嗜酸乳杆菌DSM 20079在模拟胃肠道（GI）的条件（酸和胆汁浓度）下的存活和保持活力的能力，结果显示，它在pH 2.0的环境下存活，活力下降1.2个对数周期，并且在含有0.3%胆汁的培养基中培养3小时后，45%的原始菌数存活。
