Tornóczky T, Kelényi G, Pajor L
University Medical School of Pécs, Department of Pathology, Pécs, Hungary.
Pathol Oncol Res. 1998;4(3):201-5. doi: 10.1007/BF02905249.
In virus associated diseases identification of viruses in cells can contribute to the understanding of the pathogenesis and may also help to establish the diagnosis. In the present communication, the effects of the microwave pretreatment (MWP) and that of the proteinase-K enzymatic predigestion (PKD) on EBER RNA oligonucleotide in situ hybridization (EBER-RNA-ISH) (EBER: Epstein-Barr-Encoded-(Early)-RNA) were studied. The efficacy of two EBV detecting methods, latent membrane protein-1 (LMP-1) immunohistochemistry and EBER-RNA-ISH were also compared. Our results show that microwave pretreatment enhances the intensity of the ISH signals and preserves significantly better the structure of the tissues compared with enzymatic predigestion. EBER-RNA-ISH, mainly in the nasopharyngeal carcinoma cases, showed a more frequent positivity than the immunohistochemical reaction for LMP-1, however in case of the Warthin's tumor only the LMP-1 protein was expressed.
在病毒相关疾病中，鉴定细胞中的病毒有助于理解发病机制，也可能有助于确立诊断。在本交流中，研究了微波预处理（MWP）和蛋白酶K酶消化（PKD）对EBER RNA原位杂交（EBER-RNA-ISH）（EBER：爱泼斯坦-巴尔编码（早期）RNA）的影响。还比较了两种EBV检测方法，即潜伏膜蛋白-1（LMP-1）免疫组织化学和EBER-RNA-ISH的效果。我们的结果表明，与酶消化相比，微波预处理增强了ISH信号强度，并显著更好地保留了组织结构。EBER-RNA-ISH主要在鼻咽癌病例中显示出比LMP-1免疫组化反应更频繁的阳性，但在沃辛瘤病例中仅表达LMP-1蛋白。
