Baek S C, Chae H J, Houh D, Byun D G, Cho B K
Department of Dermatology, School of Medicine, The Catholic University of Korea, Seoul, South Korea.
Int J Dermatol. 1998 Sep;37(9):682-6. doi: 10.1046/j.1365-4362.1998.00517.x.
Onychomycosis, a fungal nail infection, has become one of the most important dermatophytoses. Unfortunately, a predictably successful diagnostic approach to onychomycosis does not yet exist.
The purpose of this study was to develop a deoxyribonucleic acid (DNA)-based diagnostic method to improve the sensitivity and specificity of the detection and differentiation of the pathogenic fungi of onychomycosis.
We attempted to detect fungi in the nail using polymerase chain reaction (PCR) primer systems that were designed in conserved sequences of the small ribosomal subunit 18S-rRNA genes shared by most fungi, and differentiated between species by restriction enzyme analysis of the amplified product.
Fragments of the gene coding for 18S-rRNA were amplified successfully from medically important fungi species, but not from normal nails. Restriction fragment length polymorphism patterns using HaeIII endonuclease were sufficiently different to allow the recognition of individual species.
The PCR-restriction enzyme analysis method appears to be a more sensitive detection and identification technique for onychomycosis than conventional methods, and has considerable diagnostic value.
甲癣，一种指甲真菌感染，已成为最重要的皮肤癣菌病之一。不幸的是，目前尚无一种可预测成功的甲癣诊断方法。
本研究旨在开发一种基于脱氧核糖核酸（DNA）的诊断方法，以提高甲癣致病真菌检测和鉴别的敏感性和特异性。
我们尝试使用聚合酶链反应（PCR）引物系统检测指甲中的真菌，该引物系统设计于大多数真菌共有的小核糖体亚基18S - rRNA基因的保守序列中，并通过对扩增产物进行限制性酶切分析来区分不同菌种。
成功从医学上重要的真菌菌种中扩增出编码18S - rRNA的基因片段，但未从正常指甲中扩增出。使用HaeIII内切酶的限制性片段长度多态性模式差异足够大，能够识别各个菌种。
PCR - 限制性酶切分析方法似乎是一种比传统方法更敏感的甲癣检测和鉴定技术，具有相当大的诊断价值。
