Subramaniam V, Kirsch A K, Jovin T M
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Cell Mol Biol (Noisy-le-grand). 1998 Jul;44(5):689-700.
Scanning near-field optical microscopy (SNOM) yields high-resolution topographic and optical images and is an important technique for visualizing biological systems. We summarize the literature on SNOM of biological systems and present some of our recent applications in cellular biology. These include studies of: i) the binding of fluorescently conjugated lectins to cell surface glycoproteins on 3T3 Balb/c cells, ii) molecular interactions by fluorescence resonance energy transfer using photobleaching techniques, and iii) green fluorescent protein (GFP) expressed in bacteria.
扫描近场光学显微镜（SNOM）可产生高分辨率的形貌和光学图像，是可视化生物系统的一项重要技术。我们总结了有关生物系统SNOM的文献，并介绍了我们最近在细胞生物学中的一些应用。这些应用包括：i）荧光共轭凝集素与3T3 Balb/c细胞表面糖蛋白的结合研究，ii）使用光漂白技术通过荧光共振能量转移进行的分子相互作用研究，以及iii）细菌中表达的绿色荧光蛋白（GFP）研究。
