• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells.用于活细胞研究的混合扫描离子电导和扫描近场光学显微镜。
Biophys J. 2000 May;78(5):2675-9. doi: 10.1016/S0006-3495(00)76811-1.
2
Surface:volume relationship in cardiac myocytes studied with confocal microscopy and membrane capacitance measurements: species-dependence and developmental effects.利用共聚焦显微镜和膜电容测量研究心肌细胞的表面积与体积关系：物种依赖性和发育效应
Biophys J. 1996 Mar;70(3):1494-504. doi: 10.1016/S0006-3495(96)79711-4.
3
Observation of living cells using the atomic force microscope.使用原子力显微镜对活细胞进行观察。
Biophys J. 1993 Feb;64(2):539-44. doi: 10.1016/S0006-3495(93)81396-1.
4
Simultaneous measurement of Ca2+ and cellular dynamics: combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes.Ca2+与细胞动力学的同步测量：结合扫描离子电导和光学显微镜研究收缩心肌细胞
Biophys J. 2001 Sep;81(3):1759-64. doi: 10.1016/S0006-3495(01)75826-2.
5
Scanning ion conductance microscopy of living cells.活细胞的扫描离子电导显微镜技术
Biophys J. 1997 Aug;73(2):653-8. doi: 10.1016/S0006-3495(97)78100-1.
6
Scanning near-field optical microscopy in cell biology and microbiology.细胞生物学和微生物学中的扫描近场光学显微镜术
Cell Mol Biol (Noisy-le-grand). 1998 Jul;44(5):673-88.
7
Near-field scanning optical microscopy in cell biology and cytogenetics.细胞生物学和细胞遗传学中的近场扫描光学显微镜术
Methods Mol Biol. 2006;319:275-94. doi: 10.1007/978-1-59259-993-6_14.
8
Stroboscopic near-field imaging for the analysis of contraction of muscle cells.用于分析肌肉细胞收缩的频闪近场成像
J Microsc. 2009 Feb;233(2):269-74. doi: 10.1111/j.1365-2818.2009.03117.x.
9
Optical chemical imaging of tobacco mosaic virus in solution at 60-nm resolution.溶液中烟草花叶病毒的60纳米分辨率光学化学成像。
Biophys J. 1998 Apr;74(4):2076-9. doi: 10.1016/S0006-3495(98)77914-7.
10
High-resolution nonlinear optical imaging of live cells by second harmonic generation.通过二次谐波产生对活细胞进行高分辨率非线性光学成像。
Biophys J. 1999 Dec;77(6):3341-9. doi: 10.1016/S0006-3495(99)77165-1.

引用本文的文献

1
Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts.光刺激扫描离子电导显微镜（Opto-SICM）技术将光遗传学与扫描离子电导显微镜技术相结合，用于探测细胞间的接触。
Commun Biol. 2023 Nov 8;6(1):1131. doi: 10.1038/s42003-023-05509-3.
2
Scanning Ion Conductance Microscopy.扫描离子电导显微镜。
Chem Rev. 2021 Oct 13;121(19):11726-11768. doi: 10.1021/acs.chemrev.0c00962. Epub 2020 Dec 9.
3
Contractility assessment in enzymatically isolated cardiomyocytes.酶分离心肌细胞的收缩性评估
Biophys Rev. 2012 Sep;4(3):231-243. doi: 10.1007/s12551-012-0082-y. Epub 2012 Sep 1.
4
Potentiometric-scanning ion conductance microscopy for measurement at tight junctions.用于紧密连接测量的电位扫描离子电导显微镜。
Tissue Barriers. 2013 Oct 1;1(4):e25585. doi: 10.4161/tisb.25585. Epub 2013 Aug 9.
5
Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.扫描离子电导显微镜：活细胞生物学研究中的纳米技术。
Front Physiol. 2013 Jan 14;3:483. doi: 10.3389/fphys.2012.00483. eCollection 2012.
6
Imaging the cell surface and its organization down to the level of single molecules.对细胞表面及其组织进行成像，达到单分子水平。
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24;368(1611):20120027. doi: 10.1098/rstb.2012.0027. Print 2013 Feb 5.
7
Scanning ion conductance microscopy for studying biological samples.扫描离子电导显微镜用于研究生物样本。
Sensors (Basel). 2012 Nov 6;12(11):14983-5008. doi: 10.3390/s121114983.
8
Compartmentation of membrane processes and nucleotide dynamics in diffusion-restricted cardiac cell microenvironment.扩散受限的心肌细胞微环境中膜过程的区室化和核苷酸动力学。
J Mol Cell Cardiol. 2012 Feb;52(2):401-9. doi: 10.1016/j.yjmcc.2011.06.007. Epub 2011 Jun 16.
9
Prolonged mechanical unloading affects cardiomyocyte excitation-contraction coupling, transverse-tubule structure, and the cell surface.长时间的机械去负荷会影响心肌细胞的兴奋-收缩偶联、横管结构和细胞表面。
FASEB J. 2010 Sep;24(9):3321-9. doi: 10.1096/fj.10-156638. Epub 2010 Apr 29.
10
Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart.来自衰竭的人类和大鼠心脏的心室肌细胞中T小管的丧失及表面形态的其他变化。
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6854-9. doi: 10.1073/pnas.0809777106. Epub 2009 Apr 2.

本文引用的文献

1
Cell biological applications of scanning near-field optical microscopy (SNOM).扫描近场光学显微镜（SNOM）的细胞生物学应用。
Cell Mol Biol (Noisy-le-grand). 1998 Jul;44(5):689-700.
2
Optical chemical imaging of tobacco mosaic virus in solution at 60-nm resolution.溶液中烟草花叶病毒的60纳米分辨率光学化学成像。
Biophys J. 1998 Apr;74(4):2076-9. doi: 10.1016/S0006-3495(98)77914-7.
3
Specialized scanning ion-conductance microscope for imaging of living cells.用于活细胞成像的专用扫描离子传导显微镜。
J Microsc. 1997 Oct;188(Pt 1):17-23. doi: 10.1046/j.1365-2818.1997.2430801.x.
4
AFM review study on pox viruses and living cells.关于痘病毒与活细胞的原子力显微镜综述研究
Biophys J. 1997 Oct;73(4):2183-94. doi: 10.1016/S0006-3495(97)78250-X.
5
Scanning ion conductance microscopy of living cells.活细胞的扫描离子电导显微镜技术
Biophys J. 1997 Aug;73(2):653-8. doi: 10.1016/S0006-3495(97)78100-1.
6
Biomolecular imaging with the atomic force microscope.利用原子力显微镜进行生物分子成像。
Annu Rev Biophys Biomol Struct. 1994;23:115-39. doi: 10.1146/annurev.bb.23.060194.000555.
7
Viscoelasticity of living cells allows high resolution imaging by tapping mode atomic force microscopy.活细胞的粘弹性使得通过轻敲模式原子力显微镜进行高分辨率成像成为可能。
Biophys J. 1994 Oct;67(4):1749-53. doi: 10.1016/S0006-3495(94)80649-6.
8
The scanning ion-conductance microscope.扫描离子传导显微镜
Science. 1989 Feb 3;243(4891):641-3. doi: 10.1126/science.2464851.
9
Adriamycin cardiomyopathy in the rabbit: alterations in contractile proteins and myocyte function.兔阿霉素心肌病：收缩蛋白和心肌细胞功能的改变
Cardiovasc Res. 1990 Oct;24(10):834-42. doi: 10.1093/cvr/24.10.834.

用于活细胞研究的混合扫描离子电导和扫描近场光学显微镜。

Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells.

作者信息

Korchev Y E, Raval M, Lab M J, Gorelik J, Edwards C R, Rayment T, Klenerman D

机构信息

Division of Medicine, Imperial College School of Medicine, MRC Clinical Sciences Centre, Hammersmith Campus, London W12 0NN, United Kingdom.

出版信息

Biophys J. 2000 May;78(5):2675-9. doi: 10.1016/S0006-3495(00)76811-1.

DOI:10.1016/S0006-3495(00)76811-1
PMID:10777763
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1300856/
Abstract

We have developed a hybrid scanning ion conductance and scanning near-field optical microscope for the study of living cells. The technique allows quantitative, high-resolution characterization of the cell surface and the simultaneous recording of topographic and optical images. A particular feature of the method is a reliable mechanism to control the distance between the probe and the sample in physiological buffer. We demonstrate this new method by recording near-field images of living cells (cardiac myocytes).

摘要

我们开发了一种用于研究活细胞的混合扫描离子电导与扫描近场光学显微镜。该技术能够对细胞表面进行定量、高分辨率表征，并同时记录形貌和光学图像。此方法的一个特别之处在于，它拥有一种在生理缓冲液中控制探针与样品之间距离的可靠机制。我们通过记录活细胞（心肌细胞）的近场图像来展示这种新方法。