LaPierre L A, Casey J W, Holzschu D L
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
J Virol. 1998 Nov;72(11):8765-71. doi: 10.1128/JVI.72.11.8765-8771.1998.
Walleye dermal sarcoma (WDS) and walleye epidermal hyperplasia (WEH) are skin diseases of walleye fish that appear and regress on a seasonal basis. We report here that the complex retroviruses etiologically associated with WDS (WDS virus [WDSV]) and WEH (WEH viruses 1 and 2 [WEHV1 and WEHV2, respectively]) encode D-type cyclin homologs. The retroviral cyclins (rv-cyclins) are distantly related to one another and to known cyclins and are not closely related to any walleye cellular gene based on low-stringency Southern blotting. Since aberrant expression of D-type cyclins occurs in many human tumors, we suggest that expression of the rv-cyclins may contribute to the development of WDS or WEH. In support of this hypothesis, we show that rv-cyclin transcripts are made in developing WDS and WEH and that the rv-cyclin of WDSV induces cell cycle progression in yeast (Saccharomyces cerevisiae). WEHV1, WEHV2, and WDSV are the first examples of retroviruses that encode cyclin homologs. WEH and WDS and their associated retroviruses represent a novel paradigm of retroviral tumor induction and, importantly, tumor regression.
大眼梭鲈皮肤肉瘤（WDS）和大眼梭鲈表皮增生（WEH）是大眼梭鲈的皮肤疾病，呈季节性出现和消退。我们在此报告，与WDS病因相关的复杂逆转录病毒（WDS病毒[WDSV]）和与WEH病因相关的复杂逆转录病毒（分别为WEH病毒1和2 [WEHV1和WEHV2]）编码D型细胞周期蛋白同源物。逆转录病毒细胞周期蛋白（rv-细胞周期蛋白）彼此之间以及与已知细胞周期蛋白的关系较远，根据低严谨度Southern印迹分析，它们与任何大眼梭鲈细胞基因都没有密切关系。由于D型细胞周期蛋白的异常表达在许多人类肿瘤中都有发生，我们认为rv-细胞周期蛋白的表达可能有助于WDS或WEH的发展。为支持这一假设，我们表明在发育中的WDS和WEH中会产生rv-细胞周期蛋白转录本，并且WDSV的rv-细胞周期蛋白可诱导酵母（酿酒酵母）中的细胞周期进程。WEHV1、WEHV2和WDSV是编码细胞周期蛋白同源物的逆转录病毒的首个实例。WEH和WDS及其相关逆转录病毒代表了逆转录病毒诱导肿瘤以及重要的肿瘤消退的新模式。
