Liebermann H, Mentel R, Bauer U, Pring-Akerblom P, Dölling R, Modrow S, Seidel W
Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, D-17487 Greifswald, Germany.
J Virol. 1998 Nov;72(11):9121-30. doi: 10.1128/JVI.72.11.9121-9130.1998.
The adenovirus fiber knob causes the first step in the interaction of adenovirus with cell membrane receptors. To obtain information on the receptor binding site(s), the interaction of labeled cell membrane proteins to synthetic peptides covering the adenovirus type 3 (Ad3) fiber knob was studied. Peptide P6 (amino acids [aa] 187 to 200), to a lesser extent P14 (aa 281 to 294), and probably P11 (aa 244 to 256) interacted specifically with cell membrane proteins, indicating that these peptides present cell receptor binding sites. Peptides P6, P11, and P14 span the D, G, and I beta-strands of the R-sheet, respectively. The other reactive peptides, P2 (aa 142 to 156), P3 (aa 153 to 167), and P16 (aa 300 to 319), probably do not present real receptor binding sites. The binding to these six peptides was inhibited by Ad3 virion and was independent of divalent cations. We have also screened the antigenic epitopes on the knob with recombinant Ad3 fiber, recombinant Ad3 fiber knob, and Ad3 virion-specific antisera by enzyme-linked immunosorbent assay. The main antigenic epitopes were presented by P3, P6, P12 (aa 254 to 269), P14, and especially the C-terminal P16. Peptides P14 and P16 of the Ad3 fiber knob were able to inhibit Ad3 infection of cells.
腺病毒纤维蛋白的球状结构域是腺病毒与细胞膜受体相互作用的第一步。为了获取有关受体结合位点的信息，研究了标记的细胞膜蛋白与覆盖腺病毒3型（Ad3）纤维蛋白球状结构域的合成肽之间的相互作用。肽P6（氨基酸[aa]187至200），其次是P14（aa281至294），可能还有P11（aa244至256）与细胞膜蛋白特异性相互作用，表明这些肽呈现细胞受体结合位点。肽P6、P11和P14分别跨越R片层的D、G和Iβ链。其他反应性肽P2（aa142至156）、P3（aa153至167）和P16（aa300至319）可能不呈现真正的受体结合位点。与这六种肽的结合被Ad3病毒体抑制，且与二价阳离子无关。我们还通过酶联免疫吸附测定法，用重组Ad3纤维、重组Ad3纤维球状结构域和Ad3病毒体特异性抗血清筛选了球状结构域上的抗原表位。主要抗原表位由P3、P6、P12（aa254至269）、P14，特别是C末端的P16呈现。Ad3纤维球状结构域的肽P14和P16能够抑制Ad3对细胞的感染。
