Mayr G A, Freimuth P
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
J Virol. 1997 Jan;71(1):412-8. doi: 10.1128/JVI.71.1.412-418.1997.
The receptors on human cells which mediate adsorption of adenoviruses have not been identified. We found that murine A9 cells and Chinese hamster ovary (CHO) cells failed to bind significant levels of radiolabeled adenovirus type 2 (Ad2) virions but that derivatives of these cells carrying human chromosome 21 exhibited high levels of virus binding that was specific for the viral fiber protein. G418-resistant A9 cell transformants expressing Ad2 receptors were detected at a frequency of about 10(-4) following cotransfection with high-molecular-weight DNAs from mouse cells containing human chromosome 21 and plasmid DNA containing a neomycin resistance gene. The Ad2 receptors on the transformed A9 cells were similar to those on human cells with respect to their concentration on the cell membrane, their affinity for the viral fiber protein, and their ability to direct virus into cells along a pathway leading to delivery of the viral DNA genome into the cell nucleus. Furthermore, identical human DNA fragments were present in three independent mouse cell transformants expressing Ad2 receptors, supporting the conclusion that these human DNA fragments correspond to a gene or locus on chromosome 21 that directs the expression of Ad2 receptors in these cells.
介导腺病毒吸附的人类细胞上的受体尚未确定。我们发现，小鼠A9细胞和中国仓鼠卵巢（CHO）细胞不能结合显著水平的放射性标记的2型腺病毒（Ad2）病毒粒子，但这些携带人类21号染色体的细胞衍生物表现出高水平的病毒结合，且这种结合对病毒纤维蛋白具有特异性。在用含人类21号染色体的小鼠细胞的高分子量DNA与含新霉素抗性基因的质粒DNA共转染后，以约10^(-4)的频率检测到表达Ad2受体的G418抗性A9细胞转化体。转化的A9细胞上的Ad2受体在细胞膜上的浓度、对病毒纤维蛋白的亲和力以及将病毒沿导致病毒DNA基因组传递到细胞核的途径导入细胞的能力方面与人类细胞上的受体相似。此外，在三个独立的表达Ad2受体的小鼠细胞转化体中存在相同的人类DNA片段，支持了这些人类DNA片段对应于21号染色体上一个指导这些细胞中Ad2受体表达的基因或基因座的结论。
