Dzierzewicz Z, Cwalina B, Chodurek E, Wilczok T
Department of Biochemistry and Biophysics, Silesian Medical Academy, Sosnowiec, Poland.
Res Microbiol. 1997 Dec;148(9):785-93. doi: 10.1016/s0923-2508(97)82454-0.
The effect of metabolic activity (expressed by generation time, rate of H2S production and the activity of hydrogenase and adenosine phosphosulphate (APS)-reductase enzymes) of the 8 wild strains of Desulfovibrio desulfuricans and of their resistance to metal ions (Hg2+, Cu2+, Mn2+, Zn2+, Ni2+, Cr3+) on the rate of corrosion of carbon steel was studied. The medium containing lactate as the carbon source and sulphate as the electron acceptor was used for bacterial metabolic activity examination and in corrosive assays. Bacterial growth inhibition by metal ions was investigated in the sulphate-free medium. The rate of H2S production was approximately directly proportional to the specific activities of the investigated enzymes. These activities were inversely proportional to the generation time. The rate of microbiologically induced corrosion (MIC) of carbon steel was directly proportional to bacterial resistance to metal ions (correlation coefficient r = 0.95). The correlation between the MIC rate and the activity of enzymes tested, although weaker, was also observed (r = 0.41 for APS-reductase; r = 0.69 for hydrogenase; critical value rc = 0.30, p = 0.05, n = 40).
研究了脱硫弧菌8株野生菌株的代谢活性（以世代时间、硫化氢产生速率以及氢化酶和腺苷磷酸硫酸（APS）还原酶的活性表示）及其对金属离子（Hg2+、Cu2+、Mn2+、Zn2+、Ni2+、Cr3+）的抗性对碳钢腐蚀速率的影响。以乳酸盐作为碳源和硫酸盐作为电子受体的培养基用于细菌代谢活性检测和腐蚀试验。在无硫酸盐培养基中研究了金属离子对细菌生长的抑制作用。硫化氢的产生速率大致与所研究酶的比活性成正比。这些活性与世代时间成反比。碳钢的微生物诱导腐蚀（MIC）速率与细菌对金属离子的抗性成正比（相关系数r = 0.95）。还观察到MIC速率与所测试酶的活性之间的相关性，尽管较弱（APS还原酶的r = 0.41；氢化酶的r = 0.69；临界值rc = 0.30，p = 0.05，n = 40）。
