Blais B W, Pietrzak E, Oudit D, Wilson C, Phillippe L M, Howlett J
Laboratory Services Division, Canadian Food Inspection Agency, Ottawa, Ontario, Canada.
J Food Prot. 1998 Sep;61(9):1187-90. doi: 10.4315/0362-028x-61.9.1187.
A simple dot blot enzyme immunoassay was developed to screen enrichment broth cultures for the presence of Salmonella. This unique system utilizes macroporous polyester cloth (Polymacron) with an inexpensive hemoglobin coating to provide a high-affinity adsorbent for lipopolysaccharide (LPS) antigens in test samples. Bound LPS antigens are then detected using a monoclonal antibody conjugate recognizing a core oligosaccharide epitope common to all salmonellae frequently found in foods and related samples. The entire test (not including enrichment culture) could be completed in less than 1 h. The performance of this assay was evaluated in the analysis of enrichment broth cultures from a variety of egg and dairy products, chicken carcasses, animal feeds, and food-processing plant environmental samples for the presence of Salmonella.
开发了一种简单的斑点印迹酶免疫测定法，用于筛选增菌肉汤培养物中是否存在沙门氏菌。这种独特的系统利用带有廉价血红蛋白涂层的大孔聚酯布（Polymacron），为测试样品中的脂多糖（LPS）抗原提供高亲和力吸附剂。然后使用单克隆抗体偶联物检测结合的LPS抗原，该偶联物识别在食品和相关样品中常见的所有沙门氏菌共有的核心寡糖表位。整个测试（不包括增菌培养）可在不到1小时内完成。在分析来自各种蛋类和乳制品、鸡 carcasses、动物饲料以及食品加工厂环境样品的增菌肉汤培养物中沙门氏菌的存在情况时，评估了该测定法的性能。 （注：carcasses原词有误，可能是carcasses，意为“屠体” ）
