Sola C, Horgen L, Devallois A, Rastogi N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Morne Jolivière, Pointe à Pitre, Guadeloupe.
Res Microbiol. 1998 May;149(5):349-60. doi: 10.1016/s0923-2508(98)80440-3.
Mycobacterium tuberculosis clinical isolates (113 isolates from 78 patients) were typed using IS6110-RFLP, DR-RFLP, DR-based spoligotyping and direct repetitive element PCR (DRE-PCR). The similarities among isolates were compared for each individual method. The individual matrix distance files for each method were summed and averaged, and the resulting unique distance file was analysed by the UPGMA (unweighted pair group method with arithmetic averages). Combined numerical analysis with 3 genetic markers (IS6110-RFLP, DR-RFLP and spoligotyping) was performed for all 78 clinical isolates, whereas analysis with 4 genetic markers (with the addition of DRE-PCR) was performed on the 10 main clusters described. When compared to molecular analysis based on individual markers, the molecular description based on multiple genetic markers enabled comparison of the results obtained by individual methods and the obtaining of a more accurate view of strain identity and clusters comparison. The resulting cumulative dendrogram was more accurate for studying the population structure of M. tuberculosis and may be a good tool for elucidating intraspecies genetic microevolution.
使用IS6110-RFLP、DR-RFLP、基于DR的间隔寡核苷酸分型法（spoligotyping）和直接重复元件PCR（DRE-PCR）对结核分枝杆菌临床分离株（来自78例患者的113株分离株）进行分型。对每种单独方法所获得的分离株之间的相似性进行比较。将每种方法的个体矩阵距离文件进行汇总并求平均值，然后通过非加权组平均法（UPGMA）对所得的唯一距离文件进行分析。对所有78株临床分离株进行了3种遗传标记（IS6110-RFLP、DR-RFLP和间隔寡核苷酸分型法）的联合数值分析，而对所描述的10个主要聚类进行了4种遗传标记（增加了DRE-PCR）的分析。与基于单个标记的分子分析相比，基于多个遗传标记的分子描述能够比较通过单个方法获得的结果，并能更准确地了解菌株的同一性和聚类比较情况。所得的累积树状图对于研究结核分枝杆菌的群体结构更为准确，可能是阐明种内遗传微进化的一个良好工具。
