瓜德罗普岛艾滋病患者分离出的猿分枝杆菌分子流行病学研究
A molecular epidemiological study of Mycobacterium simiae isolated from AIDS patients in Guadeloupe.
作者信息
Legrand E, Devallois A, Horgen L, Rastogi N
机构信息
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, F-97165 Pointe à Pitre-Cedex, Guadeloupe.
出版信息
J Clin Microbiol. 2000 Aug;38(8):3080-4. doi: 10.1128/JCM.38.8.3080-3084.2000.
Abstract
A molecular epidemiological study of Mycobacterium simiae strains isolated from AIDS patients in Guadeloupe was performed by the random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of DraI- or XbaI-digested bacterial DNAs. A comparison of RAPD profiles suggested a similarity of banding patterns within a group of patients (two clusters of two and three patients), but the available epidemiological and clinical information did not support this finding. PFGE, on the other hand, showed that all the patients were contaminated with individual isolates. Combined numerical analysis performed by compiling the PFGE patterns obtained after XbaI and DraI digestions of bacterial DNAs suggested the occurrence of polyclonal infection in three of nine patients. Our results do not support a common source of M. simiae infection in Guadeloupe.
摘要
通过对从瓜德罗普岛艾滋病患者中分离出的猿分枝杆菌菌株的细菌DNA进行随机扩增多态性DNA（RAPD）和脉冲场凝胶电泳（PFGE）（用DraI或XbaI酶切），开展了一项分子流行病学研究。RAPD图谱比较表明，一组患者（两例患者的一个簇和三例患者的一个簇）内的条带模式具有相似性，但现有的流行病学和临床信息并不支持这一发现。另一方面，PFGE显示所有患者均被单独的菌株污染。通过汇总细菌DNA经XbaI和DraI酶切后获得的PFGE图谱进行的联合数值分析表明，9例患者中有3例发生了多克隆感染。我们的结果不支持瓜德罗普岛存在猿分枝杆菌感染的共同来源。
相似文献
1
A molecular epidemiological study of Mycobacterium simiae isolated from AIDS patients in Guadeloupe.瓜德罗普岛艾滋病患者分离出的猿分枝杆菌分子流行病学研究
J Clin Microbiol. 2000 Aug;38(8):3080-4. doi: 10.1128/JCM.38.8.3080-3084.2000.
2
Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks.用于比较医院感染暴发中脓肿分枝杆菌菌株的随机扩增多态性DNA聚合酶链反应
J Clin Microbiol. 1997 Dec;35(12):3132-9. doi: 10.1128/jcm.35.12.3132-3139.1997.
3
Genetic diversity of mycobacterium avium recovered from AIDS patients in the caribbean as studied by a consensus IS1245-RFLP method and pulsed-field gel electrophoresis.采用一致性IS1245-RFLP方法和脉冲场凝胶电泳研究从加勒比地区艾滋病患者中分离出的鸟分枝杆菌的遗传多样性。
Res Microbiol. 2000 May;151(4):271-83. doi: 10.1016/s0923-2508(00)00147-9.
4
Evaluation of the relatedness of strains of Mycobacterium avium using pulsed-field gel electrophoresis.使用脉冲场凝胶电泳评估鸟分枝杆菌菌株的相关性。
Eur J Clin Microbiol Infect Dis. 1995 Mar;14(3):212-7. doi: 10.1007/BF02310358.
5
Mycobacterium simiae infection in a patient with acquired immunodeficiency syndrome.一名获得性免疫缺陷综合征患者的猿猴分枝杆菌感染。
Braz J Infect Dis. 2001 Dec;5(6):352-5. doi: 10.1590/s1413-86702001000600010. Epub 2003 Feb 21.
6
Description of a novel Mycobacterium simiae allelic variant isolated from Caribbean AIDS patients by PCR-restriction enzyme analysis and sequencing of hsp65 gene.通过PCR-限制性酶切分析和hsp65基因测序对从加勒比地区艾滋病患者中分离出的新型猿分枝杆菌等位基因变体的描述。
Mol Cell Probes. 2000 Dec;14(6):355-63. doi: 10.1006/mcpr.2000.0325.
7
Mycobacterium simiae infection in Israeli patients with AIDS.以色列艾滋病患者中的猿猴分枝杆菌感染。
Clin Infect Dis. 1993 Sep;17(3):508-9. doi: 10.1093/clinids/17.3.508.
8
Disseminated Mycobacterium simiae infection in patients with AIDS.艾滋病患者的播散性猿分枝杆菌感染
J Infect. 2000 Sep;41(2):143-7. doi: 10.1053/jinf.2000.0700.
9
A pulsed-field gel electrophoresis study of Mycobacterium fortuitum in a Caribbean setting underlines high genetic diversity of the strains and excludes nosocomial outbreaks.一项在加勒比地区对偶然分枝杆菌进行的脉冲场凝胶电泳研究强调了菌株的高度遗传多样性，并排除了医院感染暴发情况。
Int J Med Microbiol. 2002 Jun;292(1):51-7. doi: 10.1078/1438-4221-00187.
10
DNA polymorphisms detected in Mycobacterium haemophilum by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳在嗜血性分枝杆菌中检测到的DNA多态性。
J Clin Microbiol. 1994 Apr;32(4):1083-4. doi: 10.1128/jcm.32.4.1083-1084.1994.
引用本文的文献
1
Clinical characteristics and imaging features of patients with nontuberculous mycobacteria in a tertiary care center.三级医疗中心非结核分枝杆菌患者的临床特征及影像学特征
J Clin Tuberc Other Mycobact Dis. 2021 Dec 27;26:100294. doi: 10.1016/j.jctube.2021.100294. eCollection 2022 Feb.
2
Methods for Detecting Mycobacterial Mixed Strain Infections-A Systematic Review.检测分枝杆菌混合菌株感染的方法——一项系统综述
Front Genet. 2020 Dec 21;11:600692. doi: 10.3389/fgene.2020.600692. eCollection 2020.
3
pulmonary disease in Iran: systematic review and meta-analysis.伊朗的肺部疾病：系统评价与荟萃分析。
New Microbes New Infect. 2018 Sep 22;26:118-123. doi: 10.1016/j.nmni.2018.09.008. eCollection 2018 Nov.
4
Clinical, Radiological, and Microbiological Characteristics of Mycobacterium simiae Infection in 97 Patients.97 例猿分枝杆菌感染的临床、影像学和微生物学特征。
Antimicrob Agents Chemother. 2018 Jun 26;62(7). doi: 10.1128/AAC.00395-18. Print 2018 Jul.
5
Nontuberculous mycobacteria in guadeloupe, martinique, and French Guiana from 1994 to 2012.1994年至2012年瓜德罗普岛、马提尼克岛和法属圭亚那的非结核分枝杆菌
Tuberc Res Treat. 2013;2013:472041. doi: 10.1155/2013/472041. Epub 2013 Dec 18.
6
Large-restriction-fragment polymorphism analysis of Mycobacterium chelonae and Mycobacterium terrae isolates.龟分枝杆菌和土地分枝杆菌分离株的大限制性片段多态性分析。
Appl Environ Microbiol. 2003 Jul;69(7):4337-41. doi: 10.1128/AEM.69.7.4337-4341.2003.
本文引用的文献
1
Combined numerical analysis based on the molecular description of Mycobacterium tuberculosis by four repetitive sequence-based DNA typing systems.基于四种基于重复序列的DNA分型系统对结核分枝杆菌进行分子描述的联合数值分析
Res Microbiol. 1998 May;149(5):349-60. doi: 10.1016/s0923-2508(98)80440-3.
2
Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.通过hsp65基因的PCR-限制性片段长度多态性分析快速鉴定分枝杆菌至种水平，并提出一种区分34种分枝杆菌的算法。
J Clin Microbiol. 1997 Nov;35(11):2969-73. doi: 10.1128/jcm.35.11.2969-2973.1997.
3
Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens.影响代表性细菌病原体基于扩增的DNA指纹图谱可靠性和可重复性的因素。
J Clin Microbiol. 1997 Feb;35(2):339-46. doi: 10.1128/jcm.35.2.339-346.1997.
4
Disseminated Mycobacterium simiae infection in a patient with AIDS: clinical features and treatment.艾滋病患者播散性猿分枝杆菌感染：临床特征与治疗
Clin Infect Dis. 1996 Oct;23(4):832-3. doi: 10.1093/clinids/23.4.832.
5
Mycobacterium xenopi IS1395, a novel insertion sequence expanding the IS256 family.偶发分枝杆菌IS1395，一种扩展IS256家族的新型插入序列。
Microbiology (Reading). 1996 Sep;142 ( Pt 9):2453-61. doi: 10.1099/00221287-142-9-2453.
6
Variations in DNA concentrations significantly affect the reproducibility of RAPD fingerprint patterns.DNA浓度的变化显著影响随机扩增多态性DNA指纹图谱的可重复性。
Res Microbiol. 1995 Sep;146(7):561-8. doi: 10.1016/0923-2508(96)80562-6.
7
Genetic analysis using random amplified polymorphic DNA markers.使用随机扩增多态性DNA标记进行遗传分析。
Methods Enzymol. 1993;218:704-40. doi: 10.1016/0076-6879(93)18053-f.
8
Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.通过DNA指纹图谱技术进行结核分枝杆菌菌株鉴定：标准化方法建议
J Clin Microbiol. 1993 Feb;31(2):406-9. doi: 10.1128/jcm.31.2.406-409.1993.
9
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.通过聚合酶链反应和限制性酶切分析快速鉴定分枝杆菌至种水平
J Clin Microbiol. 1993 Feb;31(2):175-8. doi: 10.1128/jcm.31.2.175-178.1993.
10
Mycobacterium simiae infection in Israeli patients with AIDS.以色列艾滋病患者中的猿猴分枝杆菌感染。
Clin Infect Dis. 1993 Sep;17(3):508-9. doi: 10.1093/clinids/17.3.508.
Zotero 插件