Legrand E, Devallois A, Horgen L, Rastogi N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, F-97165 Pointe à Pitre-Cedex, Guadeloupe.
J Clin Microbiol. 2000 Aug;38(8):3080-4. doi: 10.1128/JCM.38.8.3080-3084.2000.
A molecular epidemiological study of Mycobacterium simiae strains isolated from AIDS patients in Guadeloupe was performed by the random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of DraI- or XbaI-digested bacterial DNAs. A comparison of RAPD profiles suggested a similarity of banding patterns within a group of patients (two clusters of two and three patients), but the available epidemiological and clinical information did not support this finding. PFGE, on the other hand, showed that all the patients were contaminated with individual isolates. Combined numerical analysis performed by compiling the PFGE patterns obtained after XbaI and DraI digestions of bacterial DNAs suggested the occurrence of polyclonal infection in three of nine patients. Our results do not support a common source of M. simiae infection in Guadeloupe.
通过对从瓜德罗普岛艾滋病患者中分离出的猿分枝杆菌菌株的细菌DNA进行随机扩增多态性DNA（RAPD）和脉冲场凝胶电泳（PFGE）（用DraI或XbaI酶切），开展了一项分子流行病学研究。RAPD图谱比较表明，一组患者（两例患者的一个簇和三例患者的一个簇）内的条带模式具有相似性，但现有的流行病学和临床信息并不支持这一发现。另一方面，PFGE显示所有患者均被单独的菌株污染。通过汇总细菌DNA经XbaI和DraI酶切后获得的PFGE图谱进行的联合数值分析表明，9例患者中有3例发生了多克隆感染。我们的结果不支持瓜德罗普岛存在猿分枝杆菌感染的共同来源。
