Gebert A, Preiss G
Department of Functional and Applied Anatomy, Laboratory of Cell Biology and Electron Microscopy, Medical School of Hannover, 30623 Hannover, Germany.
J Microsc. 1998 Sep;191(3):297-302. doi: 10.1046/j.1365-2818.1998.00380.x.
A method is described for converting video signals of analog scanning electron microscopes (SEMs) into digital images of high quality. A plug-in card commercially available for personal computers is used for the on-line analog/digital conversion. A Windows application program written by the authors, together with low-level software drivers supplied with the plug-in card, allow digital images to be recorded, to be displayed simultaneously on the computer monitor and to be saved as a file in a standardized format. Compared to conventional photographic images obtained from the SEM camera system, the digital images possess superior sharpness of outline, excellent image definition, diminished noise and well-defined grey-scale tones. This method provides SEM images of high quality for less than $1000 from most older analog SEMs. In addition, the advantages of digital image processing can be applied to analog SEMs, including contrast enhancement, digital filtering and multichannel recording.
本文描述了一种将模拟扫描电子显微镜（SEM）的视频信号转换为高质量数字图像的方法。使用一种可商购的个人计算机插件卡进行在线模拟/数字转换。作者编写的Windows应用程序，连同插件卡附带的底层软件驱动程序，可实现数字图像的记录、在计算机显示器上同时显示以及以标准化格式保存为文件。与从SEM相机系统获得的传统摄影图像相比，数字图像具有轮廓清晰度更高、图像清晰度极佳、噪声减少以及灰度色调清晰的优点。该方法从大多数较旧的模拟SEM获取高质量SEM图像的成本不到1000美元。此外，数字图像处理的优势可应用于模拟SEM，包括对比度增强、数字滤波和多通道记录。
