Ying W Z, Sanders P W
Nephrology Research and Training Center, Department of Medicine, University of Alabama at Birmingham, and Department of Veterans Affairs Medical Center, 35294-0007, USA.
Kidney Int. 1998 Oct;54(4):1150-6. doi: 10.1046/j.1523-1755.1998.00117.x.
Tamm-Horsfall glycoprotein (THP) is a unique protein that is produced exclusively by cells of the thick ascending limb of Henle's loop (TALH). This study examined whether dietary salt altered renal THP production.
Male Sprague-Dawley rats were examined on days 1, 4, and 15 following placement in metabolic cages on diet that contained 0.3%, 1.0% or 8.0% NaCl. THP expression was quantified using Northern hybridization and Western blotting analysis.
An increase in dietary salt produced sustained increases in relative steady-state mRNA and protein levels of THP in the kidney. Addition of furosemide, but not chlorothiazide, to animals on the 8.0% NaCl diet further augmented steady-state mRNA levels of THP.
An increase in dietary salt and the loop diuretic, furosemide, increased expression of THP in the rat. The data support the involvement of this unique protein in the function of the TALH during changes in dietary salt. These findings also suggest that restriction of dietary salt may be beneficial in cast nephropathy in multiple myeloma and recurrent nephrolithiasis, two diseases in which THP can play an important pathogenetic role.
Tamm-Horsfall糖蛋白（THP）是一种独特的蛋白质，仅由髓袢升支粗段（TALH）细胞产生。本研究检测饮食中的盐是否会改变肾脏THP的产生。
将雄性Sprague-Dawley大鼠置于代谢笼中，给予含0.3%、1.0%或8.0%氯化钠的饮食，在第1、4和15天进行检测。使用Northern杂交和蛋白质印迹分析对THP表达进行定量。
饮食中盐的增加使肾脏中THP的相对稳态mRNA和蛋白质水平持续升高。在8.0%氯化钠饮食的动物中添加呋塞米而非氯噻嗪，可进一步提高THP的稳态mRNA水平。
饮食中盐的增加以及髓袢利尿剂呋塞米可增加大鼠THP的表达。这些数据支持这种独特蛋白质在饮食中盐变化期间TALH功能中的作用。这些发现还表明，限制饮食中的盐可能对多发性骨髓瘤的管型肾病和复发性肾结石有益，在这两种疾病中THP可发挥重要的致病作用。
