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[用于检测单纯疱疹病毒抗体的酶联免疫吸附测定超微量法的标准化及应用]

[Normalization and application of an ELISA ultramicro for the detection of herpes simplex virus antibodies].

作者信息

Ribas Antúnez M A, Vázquez Ramudo S, Laferte J, Alvarez Vega M

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1992;44(2):104-8.

PMID:9768198
Abstract

An ELISA ultramicro was normalized for detecting herpes simplex virus antibodies. A study was made on 145 samples from the Pedro Kourí Tropical Medicine Institute and the Blood Bank, both by ELISA ultramicro and immunofluorescence and ELISA, and 98% and 99% of coincidence was reached, respectively. It indicates good correspondence between these techniques.

摘要

对用于检测单纯疱疹病毒抗体的超微量酶联免疫吸附测定(ELISA)进行了标准化。对来自佩德罗·库里热带医学研究所和血库的145份样本进行了研究,分别采用超微量ELISA、免疫荧光法和ELISA检测,吻合率分别达到了98%和99%。这表明这些技术之间具有良好的一致性。

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