Yamada H, Hanaki N, Imamura A, Ueguchi C, Mizuno T
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
FEBS Lett. 1998 Sep 25;436(1):76-80. doi: 10.1016/s0014-5793(98)01103-x.
Previously, Arabidopsis thaliana was shown to possess a set of response regulators (ARR-series), which are implicated in the prokaryotic type of signal transduction mechanism, generally referred to as the His-Asp phosphorylay. Among them, ARR4 is a typical phospho-accepting response regulator, whose expression was recently demonstrated to be rapidly induced by a cytokinin-treatment of the plant. To gain insight into the presumed His-Asp phosphotransfer signaling mechanism as well as the role of ARR4 in this higher plant, in this study we adopt the widely used yeast two-hybrid system, and report the identification of an Arabidopsis protein that has an ability to interact physically with the cytokinin-inducible ARR4 response regulator.
此前，已证明拟南芥拥有一组应答调节因子（ARR系列），它们参与原核生物类型的信号转导机制，通常称为组氨酸-天冬氨酸磷酸化。其中，ARR4是一种典型的磷酸接受应答调节因子，最近证明其表达可通过植物的细胞分裂素处理快速诱导。为深入了解推测的组氨酸-天冬氨酸磷酰转移信号转导机制以及ARR4在这种高等植物中的作用，在本研究中我们采用了广泛使用的酵母双杂交系统，并报告鉴定出一种拟南芥蛋白，它能够与细胞分裂素诱导的ARR4应答调节因子发生物理相互作用。
